Atherosclerosis (AS) Model
High-Fat Diet Induced Aortic Plaque & Plaque Stability Evaluation Platform
Model Overview
Atherosclerosis is the fundamental pathology of coronary heart disease. By feeding ApoE-/- or Ldlr-/- knockout mice with high-fat/high-cholesterol western diets, Jitian Bio provides models representing full-stage disease development, from early fatty streaks to advanced vulnerable plaques.
Experimental Specs
- Study Duration8w - 16w
- Species / StrainC57BL/6 Mouse
- Delivery FormLive / Frozen tissue
- Bio-AnalysisIHC / WB / Omics
Key Highlights & Advantages
Aorta En Face & Root Staining
Offers standardized en face Oil Red O staining of the aorta and cross-sections of the aortic root to accurately calculate plaque area.
Consistent Plaque Distribution
Lesions form reliably in the aortic arch, brachiocephalic trunk, and carotid bifurcations, ensuring high experimental reproducibility.
Custom Diet & Severity Durations
Feeding periods can be customized (8, 12, or 16 weeks) to obtain early-stage fatty streaks or advanced fibrous plaques.
Aortic Plaque & Lipid Validation Data
Representative vascular and plasma endpoints of ApoE-/- mice fed with western diet for 12 weeks:
| Endpoint | Chow Diet Control | Atherosclerosis (HFD) | Pathological Interpretation |
|---|---|---|---|
| Serum Total Cholesterol | 2.1 ± 0.3 mmol/L | 26.4 ± 3.1 mmol/L | Indicates severe hypercholesterolemia |
| Aortic Arch Plaque Area % | 0% | 28.5 ± 3.6% | Oil Red O positive area, indicating extensive lipid lesions |
| Aortic Root Plaque Area | 0 μm² | 145,000 ± 18,500 μm² | Presence of foam cells, cholesterol clefts, and necrotic core |
| Fibrous Cap Thickness | N/A | Masson Stained Cap | Used to evaluate plaque stability and rupture risk |
Customize Your Atherosclerosis Study
Jitian Bio provides end-to-end research services including animal breeding, western diet feeding, tissue staining, and lipid biochemistry panels.