Cerebral Ischemia (MCAO) Model
Standardized Middle Cerebral Artery Occlusion & Reperfusion Platform
Model Overview
The Middle Cerebral Artery Occlusion (MCAO) model is the gold standard for studying ischemic stroke, neuroprotection, and rehabilitation. Using surgical micro-filament techniques under intraoperative Laser Doppler Flowmetry (LDF) monitoring, Jitian Bio guarantees highly uniform brain infarct sizes and high post-op survival rates.
Experimental Specs
- Study Duration24h - 14d
- Species / StrainSD Rat / C57 Mouse
- Delivery FormLive / Frozen tissue
- Bio-AnalysisIHC / WB / Omics
Key Highlights & Advantages
Excellent Infarct Uniformity
Infarct volume coefficient of variation (CV) is strictly kept within 15%, resolving the historical issues of large data fluctuations in stroke models.
Real-time Cerebral Blood Flow Monitoring
Intraoperative Laser Doppler Flowmetry (LDF) monitors blood flow reduction (>80%) and recovery to ensure consistency.
High Survival & Low Complications
Refined surgical protocols minimize subarachnoid hemorrhage and post-operative weight loss, achieving >95% survival rate at 24 hours.
Standardized Surgical Validation Parameters
Phenotypic indices and neurological scores for Jitian Bio's MCAO model:
| Evaluation Dimension | Sham Group | MCAO Group (2h) | Verification Standard |
|---|---|---|---|
| Zea Longa Score (24h) | 0 (No deficit) | 2.4 ± 0.5 | Displays typical contralateral forelimb flexion and circling |
| TTC Stained Infarct Volume % | 0% | 35.8 ± 4.2% | Clear boundary defining necrotic (white) vs viable (red) tissue |
| Rotarod Latency to Fall | 180 sec (Max) | 42.3 ± 8.4 sec | Confirms severe motor and coordination impairment |
| Brain Water Content | 78.2 ± 0.4% | 82.5 ± 0.9% | Significant cerebral edema and blood-brain barrier damage |
Schedule Your MCAO Efficacy Study
Our experienced microsurgical team offers full-service research from model surgery, customized filament selection, to histological stainings.