Experimental methods
16S rRNA sequencing assay
What this technique is
- Category
- Omics
- Principle
- An amplicon sequencing assay in which the amplicon is derived from universal primers used to amplify the 16S ribosomal RNA gene from isolate bacterial genomic DNA or metagenomic DNA from a microbioal community. Resulting sequences are compared to reference 16S sequence databases to identify or classify bacteria present within a given sample.
- Input samples
- mouse fecal DNA
- Research questions
- Not provided
- Output and readout
- 扩增子序列比对参考 16S 数据库后得到菌群组成与群落结构差异（如 V4 区扩增用于组间比较）。
- Applicability
- 用于肠道菌群与代谢表型关联研究的粪便 16S rRNA 基因扩增子测序，如抗生素暴露叠加高脂饮食的 C57BL/6 研究。
- Limitations
- 仅基于 16S 基因的分类分辨率有限，种株水平鉴定与功能推断需其他方法；引物区段选择影响组成比较。
Standard concept source：OBI:0002763 · OBI · 2026-05-08
已发布模型
No published relation.