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Experimental methods

leptin assay

What this technique is

Category
Biochemistry & immunoassay
Principle
An analyte assay that measures the abundance of leptin
Input samples
mouse serum · mouse plasma · rat serum
Research questions
Not provided
Output and readout
血浆/血清瘦素浓度按标准曲线定量;营养状态相关的瘦素升高用于评估瘦素抵抗,配体/受体缺陷模型则用于区分两类遗传性肥胖机制。
Applicability
用于饮食性肥胖、Lep 缺陷与 Lepr 缺陷啮齿模型的循环瘦素测定,例如 ELISA 联测 leptin、adiponectin、resistin 与 insulin 的激素谱。
Limitations
不同试剂盒与稀释方案间绝对值不可直接比较;样本采集时间与禁食状态影响瘦素水平。

Standard concept source:OBI:0003202 · OBI · 2026-05-08

已发布模型

No published relation.

References

  1. leptin assay (OBI:0003202), OBI 2026-05-08
  2. Plasma hormone levels of leptin, adiponectin, resistin and insulin were measured by ELISA (PMID:19398498)
  3. Mouse leptin ELISA kit used for serum leptin (PMID:24022321)