Experimental methods
高效液相色谱(HPLC)
What this technique is
- Category
- Biochemistry & immunoassay
- Principle
- 使用高效液相色谱仪分离溶液中的化合物,再由相应检测器分析分离组分。
- Input samples
- extract · chemical solution
- Research questions
- Not provided
- Output and readout
- 输出色谱峰及相应定量结果,用于分析混合物组分或化合物纯度;与质谱联用时另提供质谱检测信息。
- Applicability
- 用于可溶性化合物的分离分析,包括研究样品提取物和合成化合物的质量分析。
- Limitations
- 色谱纯度与化合物结构身份是不同结果,纯度百分比不能替代结构鉴定。
Standard concept source:OBI:0002116 · OBI · 2026-05-08
已发布模型
No published relation.
References
- HPLC (OBI:0002116; OBI 2026-05-08)
- high performance liquid chromatography assay (OBI:0002116), OBI 2026-05-08
- Older adult APOE4 mice exhibit modest immunometabolic adaptation following microglial APOE2 replacement. Neurobiology of aging. 2026
- Aildenafil citrate with superior PDE5 selectivity ameliorates cognitive and behavioral impairments in APP/PS1 Alzheimer’s disease mice: a preclinical phenotypic study. Frontiers in Pharmacology. 2026