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Experimental methods

高效液相色谱(HPLC)

What this technique is

Category
Biochemistry & immunoassay
Principle
使用高效液相色谱仪分离溶液中的化合物,再由相应检测器分析分离组分。
Input samples
extract · chemical solution
Research questions
Not provided
Output and readout
输出色谱峰及相应定量结果,用于分析混合物组分或化合物纯度;与质谱联用时另提供质谱检测信息。
Applicability
用于可溶性化合物的分离分析,包括研究样品提取物和合成化合物的质量分析。
Limitations
色谱纯度与化合物结构身份是不同结果,纯度百分比不能替代结构鉴定。

Standard concept source:OBI:0002116 · OBI · 2026-05-08

已发布模型

No published relation.

References

  1. HPLC (OBI:0002116; OBI 2026-05-08)
  2. high performance liquid chromatography assay (OBI:0002116), OBI 2026-05-08
  3. Older adult APOE4 mice exhibit modest immunometabolic adaptation following microglial APOE2 replacement. Neurobiology of aging. 2026
  4. Aildenafil citrate with superior PDE5 selectivity ameliorates cognitive and behavioral impairments in APP/PS1 Alzheimer’s disease mice: a preclinical phenotypic study. Frontiers in Pharmacology. 2026