four-vessel occlusion rat
Two-stage four-vessel occlusion produces predictable forebrain ischemia with hippocampal CA1 neuronal death, iron deposition and delayed neuronal injury while sparing the cerebellum.
Model identity
- Model name
- four-vessel occlusion rat
- Species
- rat
- Taxonomy ID
- 10116
Biological characteristics
- Phenotype stage
- CA1 injury appears after minutes of ischemia and matures over days; delayed neuronal death is a characteristic endpoint.
- Research questions
- Global forebrain ischemia; delayed neuronal death; hippocampal vulnerability; ferroptosis and neuroprotection.
- Limitations
- Global ischemia does not model focal stroke or the penumbra. The procedure requires vertebral electrocoagulation plus carotid occlusion, has a learning curve, and requires seizure and mortality control.
References
- Li Y, Zhang J. Animal models of stroke.. Animal models and experimental medicine. 2021. PMID:34557647.
- Tuo QZ, Lei P. Ferroptosis in ischemic stroke: Animal models and mechanisms.. Zoological research. 2024. PMID:39397243.
- Belenichev I, Bukhtiyarova N, Ryzhenko V, Makyeyeva L, Morozova O, Oksenych V, Kamyshnyi O. Methodological Approaches to Experimental Evaluation of Neuroprotective Action of Potential Drugs.. International journal of molecular sciences. 2024. PMID:39408802.
- NCBI Taxonomy official record: Rattus norvegicus (brown rat), Taxonomy ID 10116.
- ARRIVE 2.0 guidelines