photothrombotic cortical stroke mouse

Rose bengal photothrombosis produces a defined cortical infarct after platelet and fibrin occlusion of illuminated vessels, with blood-brain barrier breakdown and local inflammation. Lesion location and size are controlled by the illumination field, and remote hippocampal and cognitive changes can develop.

Outcome measures

存活脑组织染为红色、梗死区呈白色；以 2 mm 连续冠状切片逐孔染色成像后，测量患侧与对侧半球的梗死面积及总面积，计算患侧相对对侧的梗死面积百分比。

Experimental animals

脑切片 TTC 染色与梗死面积测定

Age: 成年（来源未报告具体周龄）
Tissue: 脑组织（2 mm 冠状切片；梗死区与存活区）

脑切片 TTC 染色与梗死面积测定

Age: 成年（来源未报告具体周龄）
Tissue: 脑组织（2 mm 冠状切片；梗死区与存活区）

Experimental procedures

TTC Staining TTC Staining The brain is removed from the skull and placed into a brain slicer mold placed in ice. Razor blades are positioned in the mold to obtain 2mm thick sections. Sections are placed in individual wells of a 24 well tissue culture dish with 500 μl of 2% TTC in 0.1M PBS. The plate is wrapped in foil and placed on a 37°C slide warmer for 30 minutes. TTC is removed and 500ul of fixative is added to each well for storage. Images of the sections are captured by Olympus S2H10 research stereo- microscope and Adobe Photoshop software. The infarct area and total area of the contralateral and ipsilateral hemispheres are measured with Scion image software (Scion Corporation). The percent infarct area is calculated as a percentage of the ipsilateral hemisphere relative to the contralateral hemisphere as previously described. [F2.JPG]]()

Model identity

Model name: photothrombotic cortical stroke mouse
Species: mouse
Taxonomy ID: 10090

Biological characteristics

Phenotype stage: The cortical lesion matures over 24 hours to days; cognitive and remote degenerative changes can appear over weeks.
Research questions: Focal cortical infarction; vascular occlusion; neuroinflammation; post-stroke cognitive impairment and repair.
Limitations: The model has little penumbra and largely direct ischemic death, does not reproduce large-vessel thromboembolism, and depends on photosensitizer dose and illumination control. Cortical lesion location limits behavioral translation.

Experimental methods

histopathology assay

Experimental protocols

脑切片 TTC 染色与梗死面积测定
Fresh brains are cut to 2-mm coronal sections, stained per well with 2% TTC in 0.1M PBS at 37°C for 30 min protected from light, stored in fixative, imaged on a stereomicroscope, and infarct percentage computed ipsilateral-versus-contralateral.
脑切片 TTC 染色与梗死面积测定
Fresh brains are cut to 2-mm coronal sections, stained per well with 2% TTC in 0.1M PBS at 37°C for 30 min protected from light, stored in fixative, imaged on a stereomicroscope, and infarct percentage computed ipsilateral-versus-contralateral.

Reagents and materials

2% TTC 染液
TTC 工作液（染色）
0.1M PBS缓冲液（TTC染色溶剂）
TTC 染色工作液的缓冲体系
组织固定液（TTC染色后保存用）
TTC 染色后的组织切片保存
组织固定液（TTC染色后保存用）
TTC 染色后的组织切片保存
0.1M PBS缓冲液（TTC染色溶剂）
TTC 染色工作液的缓冲体系
2% TTC 染液
TTC 工作液（染色）

References

Model consultation