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TNBS 相关结肠炎模型（大鼠，文献参考）

TNBS 诱导的大鼠实验性结肠炎，病理特征为过度炎症、细胞外基质重塑与氧化应激：可见严重结肠损伤、促炎细胞因子（IL-1、IL-17 等）上调、NF-κB 信号增强、MMP-2/MMP-9 表达升高与总抗氧化能力下降的氧化失衡。

Outcome measures

获得可供光学显微镜观察的 H&E 染色组织切片：细胞核蓝紫色、细胞质与结缔组织纤维粉红色，用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值，也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology
Age
成年（体重 180–230 g；来源未报告周龄）
Sex
Male
Tissue
colon（结肠）
Hematoxylin and Eosin (H&E) staining for histopathology
Age
成年（体重 180–230 g；来源未报告周龄）
Sex
Male
Tissue
colon（结肠）
Hematoxylin and Eosin (H&E) staining for histopathology
Age
成年（体重 180–230 g；来源未报告周龄）
Sex
Male
Tissue
colon（结肠）
Hematoxylin and Eosin (H&E) staining for histopathology
Age
未记载周龄，终点取材组织切片 HE 判读（离体）
Sex
Male
Tissue
colon（结肠）

Experimental procedures

脱蜡（Dewaxing） 石蜡切片置于二甲苯中脱蜡 3 次，每次 00:03:00。 复水（Rehydration） 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00，再置于 95% 乙醇中 1 次、00:03:00，随后用水漂洗。 苏木素核染色（Hematoxylin staining） 石蜡切片以苏木素染液染色 00:00:20，随后在流水下冲洗 00:01:00。 分化与返蓝（Differentiation and bluing） 石蜡切片置于返蓝液中 00:01:00，随后在流水下冲洗 00:01:00；此步使细胞核呈现蓝色。 伊红染色（Eosin staining） 石蜡切片以伊红复染 00:00:10；此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明（Dehydration and Clearing） 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00，随后置于二甲苯中 2 次、每次 00:02:00。 封片（Mounting） 石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name
TNBS 相关结肠炎模型（大鼠，文献参考）
Species
rat
Taxonomy ID
10116
Strain
Wistar Hannover
Genetic background
Wistar Hannover 为远交（封闭群）大鼠群体，多个商业机构分别维持从该群体衍生的繁育群。

Biological characteristics

Research questions
炎症性肠病的免疫与基质重塑机制；保护性与治疗性干预的时机-效应研究。
Limitations
文献参考模型，不代表公司实际保有、供应或质检；化学诱导结肠炎范式仅反映 IBD 的部分病理，不代表疾病全貌；模型结局对干预时点高度依赖（损伤后给药与损伤前给药对 MMP-2/9、NF-κB、HMGB1、iNOS 与抗氧化指标的压制幅度不同），观察窗口设计直接影响结论外推。

Experimental methods

Experimental protocols

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

References

  1. Yorulmaz H, Yorulmaz E, Özkök E, Küçük SH, Sünger E, Ebrahimi A, Yılmaz B, Altınay S. Timing-dependent protective and therapeutic effects of ethyl pyruvate in trinitrobenzene sulfonic acid-induced experimental colitis. Exp Physiol. 2026;111(8):3731-3741. DOI:10.1113/EP093786; PMID:42307112; PMCID:PMC13394157. 全文方法逐字记载 'Adult male Wistar Hannover rats weighing 180–230 g were used'，支持 strain=Wistar Hannover；摘要支持：UC 涉及过度炎症、细胞外基质重塑与氧化应激；TNBS 造成严重结肠损伤并上调促炎细胞因子、NF-κB 信号、MMP-2/9 与氧化失衡；损伤后（治疗性）给药对 MMP-2/9、NF-κB、HMGB1、iNOS 的压制与 TAC 恢复优于预防性给药（timing-dependent），支持干预时点局限。
  2. Envigo/Inotiv. The Wistar Hannover rat for carcinogenicity studies — a more effective model. Manufacturer white paper: describes commercial colonies derived from Wistar Hannover and a 96-SNP panel used to assess allelic frequencies within and between colonies. The monitoring procedure alone is not a reported finding of measured inter-colony differences.
  3. Inotiv/Envigo 官方品系页：Wistar Han outbred rats, RccHan:WIST——'The RccHan rat has been maintained from the original nucleus of 156 breeding pairs in Hannover, Germany'。支持 Wistar Han（Hannover）远交群体属性；RccHan:WIST 为特定商业群体标识，不构成实际采购来源的证据。
  4. NCBI Taxonomy record 10116: Rattus norvegicus (brown rat), rank species.
  5. Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
  6. Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
  7. Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
  8. Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
  9. Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
  10. Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
  11. Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
  12. ARRIVE 2.0 guidelines
Model consultation