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链格孢 Alternaria 鼻内激发气道炎症模型(文献参考)

单次/反复鼻内给予 A. alternata 提取物(如 100 μg/40 μL PBS)诱导固有型 Th2 气道炎症:BALF IL-5/IL-13 变化与 ILC2(CD45+CD4−Lin−CD127+CD90.2+CD25+)募集,伴嗜酸粒细胞浸润与 AHR。

Outcome measures

获得可供光学显微镜观察的 H&E 染色组织切片:细胞核蓝紫色、细胞质与结缔组织纤维粉红色,用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值,也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology

链格孢 Alternaria 鼻内激发气道炎症模型(文献参考)

Age
not reported in source; ex-vivo endpoint-collected fixed tissue section, HE readout
Tissue
lung

链格孢 Alternaria 鼻内激发气道炎症模型(文献参考)

Age
未记载周龄,终点取材组织切片 HE 判读(离体)
Tissue
lung

Experimental procedures

脱蜡(Dewaxing) 石蜡切片置于二甲苯中脱蜡 3 次,每次 00:03:00。 复水(Rehydration) 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00,再置于 95% 乙醇中 1 次、00:03:00,随后用水漂洗。 苏木素核染色(Hematoxylin staining) 石蜡切片以苏木素染液染色 00:00:20,随后在流水下冲洗 00:01:00。 分化与返蓝(Differentiation and bluing) 石蜡切片置于返蓝液中 00:01:00,随后在流水下冲洗 00:01:00;此步使细胞核呈现蓝色。 伊红染色(Eosin staining) 石蜡切片以伊红复染 00:00:10;此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明(Dehydration and Clearing) 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00,随后置于二甲苯中 2 次、每次 00:02:00。 封片(Mounting) 石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name
链格孢 Alternaria 鼻内激发气道炎症模型(文献参考)
Species
mouse
Taxonomy ID
10090

Biological characteristics

Research questions
蛋白酶活性真菌过敏原驱动的 ILC2/固有免疫气道炎症。
Limitations
文献参考模型,不代表公司实际保有、供应或质检;品系因研究而异(含免疫健全与细胞因子受体修饰品系),不指定单一背景。

Experimental methods

Experimental protocols

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

References

  1. Zhang Xiaogang, Chen Ziyang, Zuo Shaowen, Sun Hengbiao, Li Xinyao, Lu Xiao. Endothelin-A Receptor Antagonist Alleviates Allergic Airway Inflammation via the Inhibition of ILC2 Function.. Frontiers in immunology. 2022; PMID: 35222426
  2. Schmitt Pauline, Duval Anais, Camus Mylène, Lefrançais Emma, Roga Stéphane, Dedieu Cécile. TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation.. The Journal of experimental medicine. 2024; PMID: 38597952
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  6. Daines Michael, Pereira Rhea, Cunningham Aubrey, Pryor Barry, Besselsen David G, Liu Yuchen. Novel Mouse Models of Fungal Asthma.. Frontiers in cellular and infection microbiology. 2021; PMID: 34485171
  7. Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
  8. Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
  9. Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
  10. Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
  11. Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
  12. Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
  13. Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
  14. ARRIVE 2.0 guidelines