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饮食性肥胖+OVA 致敏激发哮喘复合模型(文献参考)

4 周龄起高脂饮食(D12492)16 周诱导肥胖后,以 OVA(100 μg/只)+Imject Alum 腹腔致敏并气道激发:肥胖加重气道炎症与 AHR 表型,为肥胖-哮喘共病范式。

Outcome measures

获得可供光学显微镜观察的 H&E 染色组织切片:细胞核蓝紫色、细胞质与结缔组织纤维粉红色,用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值,也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology

饮食性肥胖+OVA 致敏激发哮喘复合模型(文献参考)

Age
not reported in source; ex-vivo endpoint-collected fixed tissue section, HE readout
Tissue
lung

饮食性肥胖+OVA 致敏激发哮喘复合模型(文献参考)

Age
未记载周龄,终点取材组织切片 HE 判读(离体)
Tissue
lung

Experimental procedures

脱蜡(Dewaxing) 石蜡切片置于二甲苯中脱蜡 3 次,每次 00:03:00。 复水(Rehydration) 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00,再置于 95% 乙醇中 1 次、00:03:00,随后用水漂洗。 苏木素核染色(Hematoxylin staining) 石蜡切片以苏木素染液染色 00:00:20,随后在流水下冲洗 00:01:00。 分化与返蓝(Differentiation and bluing) 石蜡切片置于返蓝液中 00:01:00,随后在流水下冲洗 00:01:00;此步使细胞核呈现蓝色。 伊红染色(Eosin staining) 石蜡切片以伊红复染 00:00:10;此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明(Dehydration and Clearing) 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00,随后置于二甲苯中 2 次、每次 00:02:00。 封片(Mounting) 石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name
饮食性肥胖+OVA 致敏激发哮喘复合模型(文献参考)
Species
mouse
Taxonomy ID
10090
Strain
C57BL/6

Biological characteristics

Research questions
肥胖代谢状态对过敏性气道炎症严重度与治疗反应的影响。
Limitations
文献参考模型,不代表公司实际保有、供应或质检;复合构建(饮食+过敏原),不能并入单纯 OVA 模型使用数;HFD 配方因研究而异。

Experimental methods

Experimental protocols

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

References

  1. Kim Joo Young, Sohn Jung-Ho, Lee Jae-Hyun, Park Jung-Won. Obesity increases airway hyperresponsiveness via the TNF-α pathway and treating obesity induces recovery.. PloS one. 2015; PMID: 25658739
  2. Takahashi Yoshinori, Kobayashi Tetsu, D'Alessandro-Gabazza Corina N, Toda Masaaki, Fujiwara Kentaro, Okano Tomohito. Protective Role of Matrix Metalloproteinase-2 in Allergic Bronchial Asthma.. Frontiers in immunology. 2019; PMID: 31428095
  3. Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
  4. Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
  5. Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
  6. Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
  7. Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
  8. Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
  9. Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
  10. ARRIVE 2.0 guidelines