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Sprague-Dawley ICV-STZ rat

以脑室内STZ干预建立的SD大鼠AD相关认知损伤模型,可用于学习记忆及神经细胞损伤的检测。

Outcome measures

记录每次定位试验的到台潜伏期和游泳轨迹,比较相继试验中的空间定位表现。到达平台与达到时限未到台的情况按对应条件路径分别处理。

Experimental animals

大鼠 Morris 水迷宫空间定位与再学习测定
Age
成年
Sex
Female
Tissue
Sprague–Dawley ICV-STZ 大鼠;原研究为雌性成年动物。
大鼠 Morris 水迷宫空间定位与再学习测定
Age
成年
Sex
Female
Tissue
Sprague–Dawley ICV-STZ 大鼠;原研究为雌性成年动物。

Experimental procedures

检查摄像机设置 检查摄像机设置。 将 Atlantis 自动升降平台置于水池,确认升降运动正常,避免平台上下移动时产生水波 将 Atlantis 自动升降平台置于水池,确认升降运动正常,避免平台上下移动时产生水波。 将平台放置在与前一天不同的位置 将平台放置在与前一天不同的位置。 实验第一天不执行与前一天位置比较的要求 实验第一天不执行与前一天位置比较的要求。 启动 Bonsai 工作流,确认以 30 fps 记录视频 启动 Bonsai 工作流,确认以 30 fps 记录视频。 降下平台,使动物暂时无法使用平台 降下平台,使动物暂时无法使用平台。 将动物放在所选起点,面向水池中心 将动物放在所选起点,面向水池中心。 轻放动物入水,避免抛掷和溅水 轻放动物入水,避免抛掷和溅水。 释放动物且操作者离开实验区域时,启动计时器或计算机跟踪程序 释放动物且操作者离开实验区域时,启动计时器或计算机跟踪程序。 经过 1 分钟 后,按下按钮升起平台 经过 1 分钟 后,按下按钮升起平台。 动物到达并接触平台时停止计时;本次试验时限为 2 分钟 Choose the matching condition: Platform — Rats will find the platform. They will do this in two minutes or less; Not platform — If the rat does not find the platform within 2 minutes. 动物到达并接触平台时停止计时;本次试验时限为 2 分钟。 返回取出动物,放入笼内擦干 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 返回取出动物,放入笼内擦干。 间隔 30 分钟 后开始第 2 次试验 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 间隔 30 分钟 后开始第 2 次试验。 升起平台,使动物能够登台,但平台仍不可直接看见 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 升起平台,使动物能够登台,但平台仍不可直接看见。 将动物放在所选起点,面向水池中心;每次试验随机选择不同的起点 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 将动物放在所选起点,面向水池中心;每次试验随机选择不同的起点。 轻放动物入水,避免抛掷和溅水 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 轻放动物入水,避免抛掷和溅水。 释放动物且操作者离开实验区域时,启动计时器或计算机跟踪程序 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 释放动物且操作者离开实验区域时,启动计时器或计算机跟踪程序。 动物到达并接触平台,或经过 1 分钟 时停止计时 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Choose the matching condition: Platform — Rats will find the platform. They will do this in 1 minute or less; Not platform — If the rat does not find the platform within 1 minute 动物到达并接触平台,或经过 1 分钟 时停止计时。 返回取出动物,放入笼内擦干 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Platform — Rats will find the platform. They will do this in 1 minute or less 返回取出动物,放入笼内擦干。 间隔 5 分钟 后进行下一次试验 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Platform — Rats will find the platform. They will do this in 1 minute or less 间隔 5 分钟 后进行下一次试验。 将大鼠放在平台上 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 将大鼠放在平台上。 离开实验区域 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 离开实验区域。 等待 10 秒 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 等待 10 秒。 返回取出动物,放入笼内擦干并等待 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 返回取出动物,放入笼内擦干并等待。 间隔 5 分钟 后进行下一次试验 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 间隔 5 分钟 后进行下一次试验。 将大鼠放在平台上 Condition: Not platform — If the rat does not find the platform within 2 minutes. 将大鼠放在平台上。 离开实验区域 Condition: Not platform — If the rat does not find the platform within 2 minutes. 离开实验区域。 等待 10 秒 Condition: Not platform — If the rat does not find the platform within 2 minutes. 等待 10 秒。 返回取出动物,放入笼内擦干并等待 Condition: Not platform — If the rat does not find the platform within 2 minutes. 返回取出动物,放入笼内擦干并等待。 间隔 30 分钟 后开始第 2 次试验 Condition: Not platform — If the rat does not find the platform within 2 minutes. 间隔 30 分钟 后开始第 2 次试验。 升起平台,使动物能够登台,但平台仍不可直接看见 Condition: Not platform — If the rat does not find the platform within 2 minutes. 升起平台,使动物能够登台,但平台仍不可直接看见。 将动物放在所选起点,面向水池中心;每次试验使用不同的起点 Condition: Not platform — If the rat does not find the platform within 2 minutes. 将动物放在所选起点,面向水池中心;每次试验使用不同的起点。 轻放动物入水,避免抛掷和溅水 Condition: Not platform — If the rat does not find the platform within 2 minutes. 轻放动物入水,避免抛掷和溅水。 释放动物且操作者离开实验区域时,启动计时器或计算机跟踪程序 Condition: Not platform — If the rat does not find the platform within 2 minutes. 释放动物且操作者离开实验区域时,启动计时器或计算机跟踪程序。 动物到达并接触平台时停止计时;本次试验时限为 1 分钟 Condition: Not platform — If the rat does not find the platform within 2 minutes. 动物到达并接触平台时停止计时;本次试验时限为 1 分钟。

Model identity

Model name
Sprague-Dawley ICV-STZ rat
Species
rat
Strain
Sprague-Dawley

Biological characteristics

Research questions
研究星形胶质细胞、AQP4极化分布、Aβ清除与认知功能之间的联系,以及候选干预对这些表型的影响。
Limitations
属于化学诱导模型,不能直接等同于自发或遗传性AD的自然病程。

Experimental methods

Experimental protocols

  • 大鼠 Morris 水迷宫空间定位与再学习测定

    A rat water-maze procedure using an automated Atlantis platform and distal cues, with a new platform location each day. Preserve the separate platform-found and platform-not-found paths, and record escape latency and swim trajectories across the successive trials.

  • 大鼠 Morris 水迷宫空间定位与再学习测定

    A rat water-maze procedure using an automated Atlantis platform and distal cues, with a new platform location each day. Preserve the separate platform-found and platform-not-found paths, and record escape latency and swim trajectories across the successive trials.

Reagents and materials

References

  1. High-intensity interval training ameliorates Alzheimer's disease-like pathology by regulating astrocyte phenotype-associated AQP4 polarization
  2. Maroto AS, Ugarte Perez E, Cerdán Cerdá A, De Santis S. Morris Water Maze. protocols.io, published 2026-03-26, v1. DOI:10.17504/protocols.io.e6nvwn832vmk/v1.
  3. Early Neuroprotective Effects of Erucic Acid and Gentisic Acid on Hippocampal Glutamate Concentrations and Alzheimer-like Molecular Alterations in an Intracerebroventricular Streptozotocin Rat Model. PMID:42797438; DOI:10.3390/ph19091392. Model/assay applicability; not exact replication of the separate protocols.io procedure.
  4. Multimodal Imaging in Rat Model Recapitulates Alzheimer's Disease Biomarkers Abnormalities. PMID:29097597; DOI:10.1523/JNEUROSCI.1346-17.2017. Model/assay applicability; not exact replication of the separate protocols.io procedure.
  5. Anxiety-like behavior as an early endophenotype in the TgF344-AD rat model of Alzheimer’s disease.. PMID:29107184; DOI:10.1016/j.neurobiolaging.2017.09.024. Model/assay applicability; not exact replication of the separate protocols.io procedure.
  6. Schisandrin ameliorates cognitive deficits, endoplasmic reticulum stress and neuroinflammation in streptozotocin (STZ)-induced Alzheimer’s disease rats. PMID:32336744; DOI:10.1538/expanim.19-0146. Model/assay applicability; not exact replication of the separate protocols.io procedure.
  7. ARRIVE 2.0 guidelines