Reagents
RNA 纯化用 70% 乙醇
- Composition
- 70 ml 无水乙醇与 30 ml 无 RNase 水。
- Formulation
- 70%(v/v)乙醇水溶液。
- Quality standard
- 用无 RNase 水配制,供 RNA 柱纯化使用。
Published protocol versions
- 脑组织总 RNA 提取与柱上 DNA 去除 · 加入乙醇 · RNA 柱结合用乙醇 与水相等体积,400–600 µl · 70% 乙醇,以无 RNase 水配制。
Source version:protocols.io · vv1 · David A Bernlohr; Hector Martell Martinez · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Wistar ICV-STZ rat · Verified applicability · 阿尔兹海默症 (AD) 模型(研究脑能量代谢、神经损伤与认知功能的联系,评估候选干预对相关表型的影响。;Verified applicability)