Reagents
phosphate buffered saline
- Composition
- sodium chloride, potassium chloride, phosphate salts
- Formulation
- aqueous buffer
- Quality standard
- phosphate-buffered saline
- Use information
- cardiac flush and tissue washing
Published protocol versions
- 小鼠主动脉根与头臂动脉取材及切片制备 · Stick the 10 ml syringe into the apex of the left ventricle and flush the blood from the mouse with 10 ml of PBS. · cardiac flush 10 ml PBS for the first flush; 3 ml and 2 ml for subsequent flushes · phosphate-buffered saline
Source version：protocols.io · vv1 · Jan Breslow; Daniel Teupser · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Ldlr⁻⁻ 动脉粥样硬化模型 · Verified applicability · 动脉粥样硬化模型（Ldlr 缺失模型用于膳食相关高胆固醇血症及动脉粥样硬化研究。；Verified applicability）ApoE-deficient mouse · Verified applicability · 动脉粥样硬化模型（ApoE 缺失导致自发高胆固醇血症和主动脉粥样硬化病变，用于斑块形成与进展研究。；Verified applicability）ApoE-deficient C57BL/6J mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6J 背景 ApoE 缺失模型用于饮食加速的主动脉斑块形成与进展研究。；Verified applicability）ApoE-deficient C57BL/6 mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6 背景 ApoE 缺失模型用于主动脉粥样硬化病变形成研究。；Verified applicability）LDL receptor-deficient mouse · Verified applicability · 动脉粥样硬化模型（LDL 受体缺失模型用于 LDL 驱动的高胆固醇血症和饮食诱导动脉粥样硬化研究。；Verified applicability）LDL receptor-deficient C57BL/6 mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6 背景 LDL 受体缺失模型用于致动脉粥样硬化饲料诱导的斑块研究。；Verified applicability）LDL receptor-deficient C57BL/6J mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6J 背景 LDL 受体缺失模型用于 LDL 驱动动脉粥样硬化和降脂干预研究。；Verified applicability）High-fat diet-fed C57BL/6 mouse · Verified applicability · 动脉粥样硬化模型（高脂饲料喂养 C57BL/6 小鼠用于饮食诱导代谢紊乱和早期血管表型研究。；Verified applicability） · 代谢疾病（高脂饮食诱导肥胖与糖耐量受损的代谢表型研究；C57BL/6 背景下的体重、脂肪蓄积、葡萄糖耐量与胰岛素敏感性评价。；Verified applicability） · 2型糖尿病 (T2D)（Does loss of CD226 alter high-fat diet-induced obesity and adipose tissue macrophage inflammation?; Wild-type C57BL/6 littermates fed a 60% fat HFD for 16 weeks starting at 5-6 weeks of age；Verified applicability）High-fat diet-fed mouse · Verified applicability · 动脉粥样硬化模型（高脂饲料喂养小鼠用于饮食诱导高脂血症和血管炎症研究。；Verified applicability）PCSK9-AAV hypercholesterolemic mouse · Verified applicability · 动脉粥样硬化模型（PCSK9-AAV 高胆固醇血症小鼠用于成年动物快速诱导高胆固醇血症和斑块研究。；Verified applicability）ApoE3-Leiden mouse · Verified applicability · 动脉粥样硬化模型（ApoE3-Leiden 小鼠用于 APOE 变体与饮食共同作用下的动脉粥样硬化研究。；Verified applicability）ApoE-deficient Fbn1 C1039G+/- mouse · Verified applicability · 动脉粥样硬化模型（ApoE 缺失联合 Fbn1 C1039G 突变用于斑块不稳定和弹性纤维断裂研究。；Verified applicability）ApoE⁻⁻ 动脉粥样硬化模型 · Verified applicability · 动脉粥样硬化模型（ApoE 缺失模型用于高胆固醇血症及动脉粥样硬化病变研究。；Verified applicability） · 代谢疾病（ApoE⁻⁻ 代谢-动脉粥样硬化模型；Candidate (not internally validated)） - 小鼠主动脉根与头臂动脉病变油红 O 染色及面积定量 · **Preparation of Aortic Root and Brachiocephalic Artery for Lesion Quantitation:** · cardiac flush 10 ml PBS for the first flush; 3 ml and 2 ml for subsequent flushes · phosphate-buffered saline
Source version：protocols.io · vv1 · Daniel Teupser; Jan Breslow · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Ldlr⁻⁻ 动脉粥样硬化模型 · Verified applicability · 动脉粥样硬化模型（Ldlr 缺失模型用于膳食相关高胆固醇血症及动脉粥样硬化研究。；Verified applicability）ApoE⁻⁻ 动脉粥样硬化模型 · Verified applicability · 动脉粥样硬化模型（ApoE 缺失模型用于高胆固醇血症及动脉粥样硬化病变研究。；Verified applicability） · 代谢疾病（ApoE⁻⁻ 代谢-动脉粥样硬化模型；Candidate (not internally validated)）ApoE-deficient mouse · Verified applicability · 动脉粥样硬化模型（ApoE 缺失导致自发高胆固醇血症和主动脉粥样硬化病变，用于斑块形成与进展研究。；Verified applicability）ApoE-deficient C57BL/6J mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6J 背景 ApoE 缺失模型用于饮食加速的主动脉斑块形成与进展研究。；Verified applicability）ApoE-deficient C57BL/6 mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6 背景 ApoE 缺失模型用于主动脉粥样硬化病变形成研究。；Verified applicability）LDL receptor-deficient mouse · Verified applicability · 动脉粥样硬化模型（LDL 受体缺失模型用于 LDL 驱动的高胆固醇血症和饮食诱导动脉粥样硬化研究。；Verified applicability）LDL receptor-deficient C57BL/6 mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6 背景 LDL 受体缺失模型用于致动脉粥样硬化饲料诱导的斑块研究。；Verified applicability）LDL receptor-deficient C57BL/6J mouse · Verified applicability · 动脉粥样硬化模型（C57BL/6J 背景 LDL 受体缺失模型用于 LDL 驱动动脉粥样硬化和降脂干预研究。；Verified applicability）High-fat diet-fed C57BL/6 mouse · Verified applicability · 动脉粥样硬化模型（高脂饲料喂养 C57BL/6 小鼠用于饮食诱导代谢紊乱和早期血管表型研究。；Verified applicability） · 代谢疾病（高脂饮食诱导肥胖与糖耐量受损的代谢表型研究；C57BL/6 背景下的体重、脂肪蓄积、葡萄糖耐量与胰岛素敏感性评价。；Verified applicability） · 2型糖尿病 (T2D)（Does loss of CD226 alter high-fat diet-induced obesity and adipose tissue macrophage inflammation?; Wild-type C57BL/6 littermates fed a 60% fat HFD for 16 weeks starting at 5-6 weeks of age；Verified applicability）High-fat diet-fed mouse · Verified applicability · 动脉粥样硬化模型（高脂饲料喂养小鼠用于饮食诱导高脂血症和血管炎症研究。；Verified applicability）PCSK9-AAV hypercholesterolemic mouse · Verified applicability · 动脉粥样硬化模型（PCSK9-AAV 高胆固醇血症小鼠用于成年动物快速诱导高胆固醇血症和斑块研究。；Verified applicability）ApoE3-Leiden mouse · Verified applicability · 动脉粥样硬化模型（ApoE3-Leiden 小鼠用于 APOE 变体与饮食共同作用下的动脉粥样硬化研究。；Verified applicability）ApoE-deficient Fbn1 C1039G+/- mouse · Verified applicability · 动脉粥样硬化模型（ApoE 缺失联合 Fbn1 C1039G 突变用于斑块不稳定和弹性纤维断裂研究。；Verified applicability）