跳至主要内容

Akita C57BL/6 mouse

Ins2突变相关胰岛素缺乏、高血糖及糖尿病肾脏损伤。

结局指标

340 nm吸光度变化对应的葡萄糖浓度。

实验动物

血液葡萄糖浓度酶法测定
组织
blood glucose assay samples

实验步骤

Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.

模型身份

模型名称
Akita C57BL/6 mouse
物种
mouse
品系
C57BL/6
等位基因
Ins2 Akita

生物学特性

适用研究问题
胰岛内质网应激、糖尿病及糖尿病肾病。

实验方法

实验方案

配套试剂与材料

参考文献

  1. Future Perspective of Diabetic Animal Models. Endocrine, Metabolic & Immune Disorders Drug Targets. 2020
  2. Mouse models of type 1 diabetes and their use in skeletal research. Current opinion in endocrinology, diabetes, and obesity. 2022
  3. Extraction identity record b11accb0b076c72dace7dedba0f9e02620f05f93b91857af800ef6b4e09474fb
  4. Future Perspective of Diabetic Animal Models. PMID:31241444
  5. protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  6. ARRIVE 2.0 官方规范