跳至主要内容

NOD/ShiLtJ mouse

NOD/ShiLtJ mice develop spontaneous autoimmune insulitis and beta-cell destruction, leading to insulin-deficient diabetes.

结局指标

340 nm吸光度变化对应的葡萄糖浓度。

实验动物

血液葡萄糖浓度酶法测定
组织
blood glucose assay samples

实验步骤

Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.

模型身份

模型名称
NOD/ShiLtJ mouse
物种
mouse
品系
NOD/ShiLtJ

生物学特性

模型局限
Disease incidence is sex- and colony-dependent; the model does not reproduce the full heterogeneity of human type 1 diabetes.

实验方法

实验方案

配套试剂与材料

参考文献

  1. Wong FS, Pearson JA, Wen L. Is the NOD mouse a good model for type 1 diabetes? Diabetologia. 2025.
  2. protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  3. ARRIVE 2.0 官方规范