high-fat diet-induced obesity mouse
高脂饮食相关肥胖及代谢负荷模型。
结局指标
340 nm吸光度变化对应的葡萄糖浓度。
实验动物
血液葡萄糖浓度酶法测定
- 组织
- blood glucose assay samples
实验步骤
Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.
模型身份
- 模型名称
- high-fat diet-induced obesity mouse
- 物种
- mouse
生物学特性
- 适用研究问题
- 饮食相关肥胖与代谢表型。
- 模型局限
- 饲料组成及对照饲料的选择会影响组间比较。
实验方法
实验方案
- 血液葡萄糖浓度酶法测定
以己糖激酶及葡萄糖-6-磷酸脱氢酶偶联反应测定葡萄糖,使用Cobas Mira Plus读取340 nm吸光度变化。
配套试剂与材料
- 葡萄糖酶法检测试剂(Cliniqa R84682)
葡萄糖酶法反应试剂
- 多分析物校准品(Cliniqa R60010)
分析仪校准
- 定值质控血清1(Cliniqa R83082)
质控血清1
- 定值质控血清2(Cliniqa R83083)
质控血清2
参考文献
- Extraction identity record 4453a94ed03c5e4a6f6fe63663c5c85d432f4ff44d851f5ce45f3ad91b2aba7c
- Considerations and guidelines for mouse metabolic phenotyping in diabetes research. PMID:29143855
- protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- ARRIVE 2.0 官方规范