实验方法
cell count assay
方法说明
- 方法分类
- 样本采集与处理
- 技术原理
- BAL 回收液总细胞计数并经 Wright-Giemsa/流式/自动分类计数嗜酸粒细胞、中性粒细胞、淋巴细胞与巨噬细胞比例。
- 输入样本
- bronchoalveolar lavage fluid
- 可回答的研究问题
- 过敏性/非过敏性气道炎症模型中该读定的实际应用。
- 输出与判读
- 细胞绝对数与分类百分比。
- 适用条件
- 过敏性气道炎症细胞学核心终点。
- 局限
- 取材体积与回收率影响绝对计数；分类方法（镜检/流式/自动）间定义不同。 。不同研究设定的判定阈值与报告单位并不一致。
- 检测指标
- BAL cell subsets
已发布模型
没有已发布的关联。
参考文献
- Chang Yoon-Seok, Kim Yoon-Keun, Kim Tae-Bum, Kang Hye-Ryun, Kim Sun-Sin, Bahn Joon-Woo. Airway inflammation and allergen specific IgE production may persist longer than airway hyperresponsiveness in mice.. Journal of Korean medical science. 2004; PMID: 14966344
- Lloyd Clare M. Building better mouse models of asthma.. Current allergy and asthma reports. 2007; PMID: 17448336
- Peng Juan, Yang Xuexian O, Chang Seon Hee, Yang Jiong, Dong Chen. IL-23 signaling enhances Th2 polarization and regulates allergic airway inflammation.. Cell research. 2010; PMID: 19935773
- Frush Sarah, Li Zhuowei, Potts Erin N, Du Wanglei, Eu Jerry P, Garantziotis Stavros. The role of the extracellular matrix protein mindin in airway response to environmental airways injury.. Environmental health perspectives. 2011; PMID: 21684833
- Horka Helena, Staudt Valérie, Klein Matthias, Taube Christian, Reuter Sebastian, Dehzad Nina. The tick salivary protein sialostatin L inhibits the Th9-derived production of the asthma-promoting cytokine IL-9 and is effective in the prevention of experimental asthma.. Journal of immunology (Baltimore, Md. : 1950). 2012; PMID: 22327077
- Luks Vanessa, Burkett Andrew, Turner Lucy, Pakhale Smita. Effect of physical training on airway inflammation in animal models of asthma: a systematic review.. BMC pulmonary medicine. 2013; PMID: 23617952