albumin concentration assay

What this technique is

Category

Biochemistry & immunoassay

Principle

An albumin assay that measures the concentration of albumin in a specimen.

Input samples

mouse urine · rat urine

Research questions

Not provided

Output and readout

以标准曲线报告样品中白蛋白浓度（如 mg/l）。与尿肌酐测定联用时可换算为白蛋白/肌酐比值，比值须分别测定两个组成物。

Applicability

适用于小鼠、大鼠尿液白蛋白浓度定量；其他基质（血清、组织裂解液）需按各自稀释与验证条件。

Limitations

单一白蛋白浓度不等于完整的白蛋白/肌酐比值（uACR）；染料结合法受尿液其他蛋白影响，须遵循同批标准曲线与说明书判读。

Standard concept source：OBI:0003148 · OBI · 2026-05-08

已发布模型

• db/db (Lepr^db)
• streptozotocin-induced diabetes C57BL/6 mouse
• db/db C57BLKS mouse
• high-fat diet plus streptozotocin-induced type 2 diabetes C57BL/6J mouse
• streptozotocin-induced diabetes mouse
• ob/ob BTBR mouse
• db/db mouse
• db/db mouse (C57BL/6 background)
• uninephrectomized db/db mouse (C57BLKS background)

Published protocol versions

• 小鼠/大鼠尿白蛋白定量（Albumin Blue 580 染料结合微量板法）

  Source version：protocols.io · vv1 · Peter Havel · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

References

1. albumin concentration assay (OBI:0003148), OBI 2026-05-08
2. Rodent diabetic kidney studies quantifying urinary albumin with urine creatinine normalization (PMID 27585918; 26080907)
3. Mouse models of diabetic nephropathy (AMDCC phenotyping guidance on albuminuria measurement) (PMID 19729434)