Experimental methods
Sanger sequencing
What this technique is
- Category
- Biochemistry & immunoassay
- Principle
- A DNA sequencing assay which rely on the use of dideoxynucleotides used in 4 distinct sequencing reaction on the same DNA sample. The dideoxynucleotides, once incorporated in the complementary DNA strand being synthesized by the DNA polymerase prevent any further chain elongation. The newly generated sequences are resolved on a polyacrylamide gel using electrophoresis and labels (either fluorochrome or radioactivity) are used to determine the nucleotide present at a given position
- Input samples
- DNA
- Research questions
- Not provided
- Output and readout
- 输出待测 DNA 的核苷酸序列，可用于核对靶区序列与序列差异。
- Applicability
- DNA 核苷酸序列测定。
- Limitations
- 输出为 DNA 序列信息，不直接提供基因表达量或变异功能效应。
Standard concept source：OBI:0000695 · OBI · 2026-05-08
已发布模型
No published relation.