实验方法
Sanger sequencing
方法说明
- 方法分类
- 生化及免疫检测
- 技术原理
- A DNA sequencing assay which rely on the use of dideoxynucleotides used in 4 distinct sequencing reaction on the same DNA sample. The dideoxynucleotides, once incorporated in the complementary DNA strand being synthesized by the DNA polymerase prevent any further chain elongation. The newly generated sequences are resolved on a polyacrylamide gel using electrophoresis and labels (either fluorochrome or radioactivity) are used to determine the nucleotide present at a given position
- 输入样本
- DNA
- 可回答的研究问题
- 未提供
- 输出与判读
- 输出待测 DNA 的核苷酸序列，可用于核对靶区序列与序列差异。
- 适用条件
- DNA 核苷酸序列测定。
- 局限
- 输出为 DNA 序列信息，不直接提供基因表达量或变异功能效应。
已发布模型
没有已发布的关联。