alanine aminotransferase assay

What this technique is

Category

Biochemistry & immunoassay

Principle

An analyte assay that measures the abundance of alanine aminotransferase

Input samples

mouse plasma · mouse serum

Research questions

Not provided

Output and readout

以速率法或试剂盒测得血浆 ALT 活性（U/L），与同背景对照比较提示肝细胞损伤程度。

Applicability

作为肝功能与肝损伤指标用于 NASH/肝纤维化模型（如 CDAHFD 高脂饮食小鼠）及药物干预研究。

Limitations

ALT 升高不特异于某一病因；溶血与采血应激影响活性测定；不同仪器方法需同批比较。

Standard concept source：OBI:2100016 · OBI · 2026-05-08

已发布模型

• GAN 饮食相关 MASH 模型（文献参考）

Published protocol versions

• 血样 ALT 活性测定（Teco A524 动力学法）

  Source version：manufacturer · v2018-02 · Teco Diagnostics · Manufacturer package insert; no open license stated.

References

1. alanine aminotransferase assay (OBI:2100016), OBI 2026-05-08
2. Plasma ALT and AST measured as indicators of hepatic function; ALT increased by 18 weeks of HFD (PMID:23305254)
3. Plasma ALT quantified alongside hepatic lipids (PMID:28159867)