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Beta-catenin activating model(文献参考)

杂合子Ctnnb1 +/lox(ex3) Krt1-19 +/Cre动物在十二指肠和近端空肠发育超过3000个息肉,在结肠仅形成微腺瘤,见于出生后第三周。

Outcome measures

获得可供光学显微镜观察的 H&E 染色组织切片:细胞核蓝紫色、细胞质与结缔组织纤维粉红色,用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值,也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology

Beta-catenin activating model(文献参考)

Age
not reported in source; ex-vivo endpoint-collected fixed tissue section, HE readout
Tissue
intestine

Beta-catenin activating model(文献参考)

Age
未记载周龄,终点取材组织切片 HE 判读(离体)
Tissue
intestine

Experimental procedures

脱蜡(Dewaxing) 石蜡切片置于二甲苯中脱蜡 3 次,每次 00:03:00。 复水(Rehydration) 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00,再置于 95% 乙醇中 1 次、00:03:00,随后用水漂洗。 苏木素核染色(Hematoxylin staining) 石蜡切片以苏木素染液染色 00:00:20,随后在流水下冲洗 00:01:00。 分化与返蓝(Differentiation and bluing) 石蜡切片置于返蓝液中 00:01:00,随后在流水下冲洗 00:01:00;此步使细胞核呈现蓝色。 伊红染色(Eosin staining) 石蜡切片以伊红复染 00:00:10;此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明(Dehydration and Clearing) 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00,随后置于二甲苯中 2 次、每次 00:02:00。 封片(Mounting) 石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name
Beta-catenin activating model(文献参考)
Species
mouse
Taxonomy ID
10090
Genotype / zygosity
Ctnnb1 +/lox(ex3)

Biological characteristics

Phenotype stage
出生后第三周
Research questions
综述评估表达稳定化beta-catenin的Ctnnb1激活模型。
Limitations
文献参考模型,不代表公司实际保有、供应或质检。本包内无原研实际使用,依据为综述归纳;病变主体位于小肠(十二指肠和近端空肠),结肠仅形成微腺瘤,对结肠特异性致癌研究的适用性有限。

Experimental methods

Experimental protocols

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

References

  1. Stastna Monika, Janeckova Lucie, Hrckulak Dusan, Kriz Vitezslav, Korinek Vladimir. Human Colorectal Cancer from the Perspective of Mouse Models.. Genes. 2019; PMID: 31614493
  2. Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
  3. Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
  4. Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
  5. Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
  6. Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
  7. Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
  8. Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
  9. ARRIVE 2.0 guidelines