Beta-catenin activating model(文献参考)
杂合子Ctnnb1 +/lox(ex3) Krt1-19 +/Cre动物在十二指肠和近端空肠发育超过3000个息肉,在结肠仅形成微腺瘤,见于出生后第三周。
Outcome measures
获得可供光学显微镜观察的 H&E 染色组织切片:细胞核蓝紫色、细胞质与结缔组织纤维粉红色,用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值,也不提供特定分子标志物的定量结果。
Experimental animals
Beta-catenin activating model(文献参考)
- Age
- not reported in source; ex-vivo endpoint-collected fixed tissue section, HE readout
- Tissue
- intestine
Beta-catenin activating model(文献参考)
- Age
- 未记载周龄,终点取材组织切片 HE 判读(离体)
- Tissue
- intestine
Experimental procedures
脱蜡(Dewaxing) 石蜡切片置于二甲苯中脱蜡 3 次,每次 00:03:00。 复水(Rehydration) 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00,再置于 95% 乙醇中 1 次、00:03:00,随后用水漂洗。 苏木素核染色(Hematoxylin staining) 石蜡切片以苏木素染液染色 00:00:20,随后在流水下冲洗 00:01:00。 分化与返蓝(Differentiation and bluing) 石蜡切片置于返蓝液中 00:01:00,随后在流水下冲洗 00:01:00;此步使细胞核呈现蓝色。 伊红染色(Eosin staining) 石蜡切片以伊红复染 00:00:10;此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明(Dehydration and Clearing) 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00,随后置于二甲苯中 2 次、每次 00:02:00。 封片(Mounting) 石蜡切片以 Permount 封片介质加盖玻片封固。
Model identity
- Model name
- Beta-catenin activating model(文献参考)
- Species
- mouse
- Taxonomy ID
- 10090
- Genotype / zygosity
- Ctnnb1 +/lox(ex3)
Biological characteristics
- Phenotype stage
- 出生后第三周
- Research questions
- 综述评估表达稳定化beta-catenin的Ctnnb1激活模型。
- Limitations
- 文献参考模型,不代表公司实际保有、供应或质检。本包内无原研实际使用,依据为综述归纳;病变主体位于小肠(十二指肠和近端空肠),结肠仅形成微腺瘤,对结肠特异性致癌研究的适用性有限。
Experimental methods
Experimental protocols
- Hematoxylin and Eosin (H&E) staining for histopathology
Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.
Reagents and materials
- 组织学二甲苯(混合异构体)
脱蜡剂
- Ethanol
复水梯度溶剂(去除二甲苯)
- 95% 乙醇溶液
复水梯度溶剂
- 自来水(组织学染色用)
复水后漂洗用水(后续各流水冲洗共用)
- Epredia Signature Series 苏木素染液(7221)
细胞核染色
- Epredia Signature Series 返蓝液(7301)
返蓝(使细胞核转蓝)
- Epredia Signature Series 伊红 Y 染液(7111)
细胞质与结缔组织纤维复染
- Permount 组织学封片剂(Fisher SP15)
封片介质
References
- Stastna Monika, Janeckova Lucie, Hrckulak Dusan, Kriz Vitezslav, Korinek Vladimir. Human Colorectal Cancer from the Perspective of Mouse Models.. Genes. 2019; PMID: 31614493
- Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
- Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
- Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
- Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
- Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
- Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
- Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
- ARRIVE 2.0 guidelines