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动脉组织石蜡切片 H&E 染色

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Hematoxylin and eosin staining of paraffin arterial sections for histological morphology.

Scope and method

Sample：4 μm 石蜡包埋动脉组织切片

组织学分析

Reagents and materials

Procedure

  1. H&E staining

    **Dewaxing:** Paraffin sections are placed in xylene for 3 x 00:03:00

  2. H&E staining

    **Rehydration: ** Paraffin sections are placed in - 100% EtOH for 3 x 00:03:00 - 95% EtOH for 1 x 00:03:00 - Rinse with water

  3. H&E staining

    **Hematoxylin staining: ** Paraffin sections are stained with hematoxylin for 00:00:20 and rinse under running water for 00:01:00

  4. H&E staining

    **Differentiation and bluing:** Paraffin sections are placed into the bluing reagent for 00:01:00 and rinse under running water for 00:01:00 _This step results in a blue coloration of the nuclei._

  5. H&E staining

    **Eosin staining:** Parrafin sections are counterstained with Eosin for 00:00:10 _This step results in a pink coloration of the cytoplasmic components and connective tissue fibers._

  6. H&E staining

    **Dehydration and Clearing:** Paraffin sections are placed in - 95% EtOH for 00:02:00 - 100% EtOH for 2 x 00:02:00 - Xylene for 2 x 00:02:00

  7. H&E staining

    **Mounting:** Parrafin sections are mounted with a glass coverslip using Permount.

Quality control and readouts

切片厚度和脱蜡/复水时间在各批之间保持一致；苏木素和伊红染色时间按来源步骤执行；每批包含已知病变切片作为染色对照。

获得可用于兔主动脉斑块组织学分级和组成的 HE 染色切片。

References

  1. protocols.io · vv1 · Nathalia L. Oliveira; samantha.gruenheid · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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