跳至主要内容

动脉组织石蜡切片 H&E 染色

浏览实验方案

通过苏木素与伊红显示动脉组织的细胞核、细胞质及结缔组织结构。

适用范围与方法

样本：4 μm 石蜡包埋动脉组织切片

组织学分析

试剂与材料

实验流程

  1. H&E staining

    **Dewaxing:** Paraffin sections are placed in xylene for 3 x 00:03:00

  2. H&E staining

    **Rehydration: ** Paraffin sections are placed in - 100% EtOH for 3 x 00:03:00 - 95% EtOH for 1 x 00:03:00 - Rinse with water

  3. H&E staining

    **Hematoxylin staining: ** Paraffin sections are stained with hematoxylin for 00:00:20 and rinse under running water for 00:01:00

  4. H&E staining

    **Differentiation and bluing:** Paraffin sections are placed into the bluing reagent for 00:01:00 and rinse under running water for 00:01:00 _This step results in a blue coloration of the nuclei._

  5. H&E staining

    **Eosin staining:** Parrafin sections are counterstained with Eosin for 00:00:10 _This step results in a pink coloration of the cytoplasmic components and connective tissue fibers._

  6. H&E staining

    **Dehydration and Clearing:** Paraffin sections are placed in - 95% EtOH for 00:02:00 - 100% EtOH for 2 x 00:02:00 - Xylene for 2 x 00:02:00

  7. H&E staining

    **Mounting:** Parrafin sections are mounted with a glass coverslip using Permount.

质量控制与输出

切片厚度和脱蜡/复水时间在各批之间保持一致；苏木素和伊红染色时间按来源步骤执行；每批包含已知病变切片作为染色对照。

获得可用于兔主动脉斑块组织学分级和组成的 HE 染色切片。

参考文献

  1. protocols.io · vv1 · Nathalia L. Oliveira; samantha.gruenheid · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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