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小鼠低剂量链脲佐菌素多次注射糖尿病诱导

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Freshly prepared streptozotocin (7.5 mg/ml in pH 4.5 sodium citrate buffer) injected intraperitoneally at 50 mg/kg for five consecutive days in fasted, isoflurane-anesthetized mice, with hyperglycaemia confirmed at four weeks.

Scope and method

Sample：mouse (in vivo induction)

实验性疾病诱导

Reagents and materials

Procedure

  1. Prepare the buffers and solutions as described below. Please note that the STZNa-Citrate solution should be prepared imm

    Prepare the buffers and solutions as described below. Please note that the STZNa-Citrate solution should be prepared immediately prior to injection so as to avoid degradation of the STZ.

  2. Mice should be fasted prior to injection; four hours is usually sufficient.

    Mice should be fasted prior to injection; four hours is usually sufficient.

  3. Place one mouse in the Isoflurane drop jar, according to your local IACUC anesthesia standards.

    Place one mouse in the Isoflurane drop jar, according to your local IACUC anesthesia standards.

  4. Remove mouse when breathing has slowed and animal is anesthetized

    Remove mouse when breathing has slowed and animal is anesthetized

  5. Inject appropriate amount of the STZ solution IP so the final dosage is **50 mg/kg mouse**

    Inject appropriate amount of the STZ solution IP so the final dosage is **50 mg/kg mouse**

  6. Allow mouse to awaken and place back in cage

    Allow mouse to awaken and place back in cage

  7. Repeat procedure for each animal; keep in mind that Isoflurane will need to be reloaded after every third or fourth anim

    Repeat procedure for each animal; keep in mind that Isoflurane will need to be reloaded after every third or fourth animal for proper anesthesia.

  8. Each mouse should be given one injection for 5 days consecutively.

    Each mouse should be given one injection for 5 days consecutively.

  9. Supply mice with 10% sucrose water, if necessary, to avoid sudden hypoglycemia post-injection.

    Supply mice with 10% sucrose water, if necessary, to avoid sudden hypoglycemia post-injection.

  10. **Mice should be tested for sufficient levels of hyperglycemia at 4 weeks postinjection. **If any of the experimental an

    **Mice should be tested for sufficient levels of hyperglycemia at 4 weeks postinjection. **If any of the experimental animals are not sufficiently diabetic, the low dose STZ protocol should be re-administered to those mice only at week 7 post injection to obtain proper levels of hyperglycemia.

Quality control and readouts

STZ 溶液现配现用（溶液中 15-20 分钟内降解）、全程避光；注射后必要时给予 10% 蔗糖水防止急性低血糖。

输出为达到既定高血糖阈值的成模动物；本方案不主张与某一原论文的给药操作等同，仅为可采用的诱导实现。

References

  1. Low-Dose Streptozotocin Induction Protocol (mouse), protocols.io v2

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