LightCycler 480 384-well reaction plate (Roche 4729749001)

Quality standard

The source specifies Roche Diagnostics 4729749001 with 384 wells. This is a source material reference, not a company sales SKU or supply commitment.

Use information

Used for Sirt1 and GAPDH qPCR reactions on the LightCycler 480.

References

1. protocols.io v1; step protocolsio-1477109; verbatim source: LightCycler 480 Multiwell 384-plate (#4729749001, Roche Diagnostics)

Used in these protocol BOMs

• Quantification of Sirt1 transcripts in rat brain tissue (cDNA qPCR)protocol #185 · revision 3

  Source version：protocols.io · v1 · Miquel Vila · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

  Real-time quantitative PCR (qPCR)

  This reagent's uses & amounts

  • Addition of cDNA · Used for Sirt1 and GAPDH qPCR reactions on the LightCycler 480. · Not reported in the source · The source specifies Roche Diagnostics 4729749001 with 384 wells. This is a source material reference, not a company sales SKU or supply commitment.

  Associated models: 11 protocol→model record(s)1 verified · 0 candidate

  Model → research topic：1 verified · 0 candidate。These statuses apply to individual relationships, not to this reagent.

  Recorded use and applicability limits

  • Protocol → model：Wistar ICV-STZ rat：

    Using GAPDH as the internal reference, the qPCR detection phase for comparing the relative expression level of Sirt1 in rat brain tissue.

  • Protocol → model：Wistar ICV-STZ rat：

    Age：3–4 months of age

  • Protocol → model：Wistar ICV-STZ rat：

    Sex：Male

  • Protocol → model：Wistar ICV-STZ rat：

    Tissue：Brain tissue-derived cDNA.

  • Model → research topic：Wistar ICV-STZ rat → Alzheimer disease：

    Study the connections among brain energy metabolism, neural injury, and cognitive function, and evaluate the effects of candidate interventions on the relevant phenotypes.

  Show 1 model association(s)

  Wistar ICV-STZ rat · Verified applicability

  This protocol's applicability to this model

  Status：Verified applicability· Evidence type：Literature evidence· Source：PMID: 32336744; DOI: 10.1538/expanim.19-0146; The Real-time quantitative PCR section provides Sirt1/GAPDH primers and their application to brain tissue. The SYBR detection branch of protocols.io ewov1oyz2lr2/v1 was used; the PowerUp/LightCycler480 conditions differ from the original study's SY1020/Exicycler96 system, and no claim is made that the original experimental parameters or manufacturers are identical.

  Age：3–4 months of ageSex：MaleTissue：Brain tissue-derived cDNA.

  Using GAPDH as the internal reference, the qPCR detection phase for comparing the relative expression level of Sirt1 in rat brain tissue.

  Related research topics: 1 (1 verified · 0 candidate)

  • · Alzheimer disease · Verified applicability

    This model's applicability to this disease

    Status：Verified applicability· Evidence type：Literature evidence· Source：https://doi.org/10.1538/expanim.19-0146

    Study the connections among brain energy metabolism, neural injury, and cognitive function, and evaluate the effects of candidate interventions on the relevant phenotypes.

Reference, supply and purchase path

No verified catalog mapping exists for this reagent; reference status: unknown.

No listed sale configuration is published here. This states neither discontinuation nor current stock.