1.5 ml microcentrifuge tubes for RNA extraction

Quality standard

Volume 1.5 ml. The preparation step specifies three tubes per sample; additional replacement tubes in later steps must be accounted for separately.

Use information

Used for chloroform phase separation, aqueous-phase transfer and eluate collection during brain tissue RNA extraction.

References

1. protocols.io v1; step protocolsio-2097960; verbatim source: three 1.5 ml tubes

Used in these protocol BOMs

• Total RNA extraction from brain tissue with on-column DNA removalprotocol #186 · revision 3

  Source version：protocols.io · v1 · David A Bernlohr; Hector Martell Martinez · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

  Total RNA extraction

  This reagent's uses & amounts

  • Prepare consumables · Used for chloroform phase separation, aqueous-phase transfer and eluate collection during brain tissue RNA extraction. · 3 per sample during sample preparation; the total number of later replacement tubes is not reported in the source · Volume 1.5 ml. The preparation step specifies three tubes per sample; additional replacement tubes in later steps must be accounted for separately.

  Associated models: 11 protocol→model record(s)1 verified · 0 candidate

  Model → research topic：1 verified · 0 candidate。These statuses apply to individual relationships, not to this reagent.

  Recorded use and applicability limits

  • Protocol → model：Wistar ICV-STZ rat：

    Extract total RNA from brain tissue, remove residual DNA, and prepare RNA for subsequent Sirt1/GAPDH expression detection.

  • Protocol → model：Wistar ICV-STZ rat：

    Age：3–4 months of age

  • Protocol → model：Wistar ICV-STZ rat：

    Sex：Male

  • Protocol → model：Wistar ICV-STZ rat：

    Tissue：Brain tissue

  • Model → research topic：Wistar ICV-STZ rat → Alzheimer disease：

    Study the connections among brain energy metabolism, neural injury, and cognitive function, and evaluate the effects of candidate interventions on the relevant phenotypes.

  Show 1 model association(s)

  Wistar ICV-STZ rat · Verified applicability

  This protocol's applicability to this model

  Status：Verified applicability· Evidence type：Literature evidence· Source：PMID: 32336744; DOI: 10.1538/expanim.19-0146. The Real-time quantitative PCR section records the detection of Sirt1/GAPDH in rat brain tissue RNA→cDNA. The cited protocols.io source provides the complete workflow for this stage; the TRIpure/Super MMLV used in that study differs from the TRIzol/PureLink/ProtoScript II reagent system used in this instance, and no claim is made of in-house validation or consistency with the original study parameters.

  Age：3–4 months of ageSex：MaleTissue：Brain tissue

  Extract total RNA from brain tissue, remove residual DNA, and prepare RNA for subsequent Sirt1/GAPDH expression detection.

  Related research topics: 1 (1 verified · 0 candidate)

  • · Alzheimer disease · Verified applicability

    This model's applicability to this disease

    Status：Verified applicability· Evidence type：Literature evidence· Source：https://doi.org/10.1538/expanim.19-0146

    Study the connections among brain energy metabolism, neural injury, and cognitive function, and evaluate the effects of candidate interventions on the relevant phenotypes.

Reference, supply and purchase path

No verified catalog mapping exists for this reagent; reference status: unknown.

No listed sale configuration is published here. This states neither discontinuation nor current stock.