Fetal bovine serum (FBS)
- Composition
- Fetal bovine serum, cell culture medium supplement (commonly 10%).
- Use information
- Scientific research experiments; please confirm specifications and inventory with the store.
Reagents
Insulin (insulin)
- Composition
- Insulin (peptide hormone), reference entry for materials category; the 2020 guideline ITT protocol does not specify a particular insulin type/analog, manufacturer, or purity grade, and this entry does not endorse any specific commercial product.
- Use information
- Peptide hormones, used as exogenous insulin loading reagents for glucose homeostasis studies such as the insulin tolerance test (ITT) in experimental animals.
- ChEBI
- CHEBI:145810
Trypsin-EDTA digestion solution
- Composition
- 0.25% trypsin + 0.02% EDTA, for dissociation of adherent cells during subculture.
- Use information
- Scientific research experiments; please confirm specifications and inventory with the store.
Lipopolysaccharide (LPS)
- Composition
- Gram-negative bacterial endotoxin
- Use information
- Innate immune stimulation/model auxiliary tools.
Lipopolysaccharide (LPS, source not specified)
- Composition
- Lipopolysaccharide; the microbial source or serotype was not specified in this batch of the study.
- Use information
- Research cell stimulation materials; kept separate from reagent records limited to E. coli sources.
Brain section glycerol/Tris/n-propyl gallate mounting medium
- Composition
- Mounting mixture of 90% glycerol, 20 mM Tris pH 8.0, 0.5% n-propyl gallate
- Formulation
- 90% glycerol, 20 mM Tris (pH 8.0), 0.5% n-propyl gallate.
- Quality standard
- Glycerol/Tris/n-propyl gallate formulation, pH 8.0.
- Use information
- Used for mounting immunofluorescence brain sections.
Tap water (for histological staining)
- Composition
- Municipal tap water
- Use information
- Used for each water rinse and running water wash step in H&E staining of paraffin sections
Hematoxylin staining solution
- Composition
- Mayer's or Harris hematoxylin staining solution, for nuclear staining in HE staining.
- Use information
- For nuclear staining of tissue sections: paired with eosin for routine HE staining, and also used as a nuclear counterstain for Masson's trichrome, PAS, and immunohistochemical chromogenic development.
Fluorescent secondary antibody kit (Alexa Fluor)
- Composition
- Fluorescently labeled secondary antibodies such as Alexa Fluor 488/555/647 (anti-rabbit/anti-mouse/anti-chicken IgG), for IF and mIHC.
- Use information
- Scientific research experiments; please confirm specifications and inventory with the store.
Dextran sulfate sodium
- Composition
- Sodium salt of dextran sulfate (dextran sulfate sodium).
- Use information
- For studies on experimental colitis in mice.
- ChEBI
- CHEBI:191875