Technical services

Histopathology

Examine tissue pathology with section staining and immunohistochemistry.

Observe tissue morphology and lesions, including plaque section staining in atherosclerosis research.

Immunohistochemistry to examine the distribution of target markers in tissue.

Explore gene expression and protein measures with qPCR, ELISA and WB.

Quantitative PCR analysis to measure target gene expression.

Enzyme-linked immunosorbent analysis to measure target proteins in samples.

Western blot analysis to examine target protein expression.

Explore molecular changes in tissues and cells through transcriptomic data in model research.

Analyze gene expression at single-cell resolution, including hippocampal tissue in Alzheimer’s disease model research.

Sample requirements, assay scope, deliverables and current availability are confirmed for each research project.

Protocols apply a method to a defined measurement, with samples, procedures and quality controls. Available services are linked separately.

实验小鼠心脏灌注固定：解剖学研究组织标本制备
Version 3
Adult mice are anesthetized by intraperitoneal injection of ketamine (150 mg/kg) with xylazine (15 mg/kg); the chest is opened and a needle connected to a peristaltic pump is placed in the left ventricle, and a prewash solution followed by 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) is perfused. A step in the flow labels the arterial and capillary systems in vivo with DyLight 649-conjugated tomato lectin injected into the left ventricle. Dissected tissues are post-fixed at 4 °C for 18–24 h, washed in 0.1 M PBS (pH 7.2) and stored at 4 °C in PBS containing 0.1% sodium azide until used for immunohistochemistry or other microscopic analysis, yielding well-preserved tissue for anatomical studies. Institutional animal ethics approval must be obtained before performing this procedure.
眼眶后静脉丛采血 · protocol
Version 2
mouse blood
tumor challenge · protocol
Version 2
mouse tumor