跳至主要内容

spontaneously diabetic wbn/kob rat

自发性糖尿病相关的周围神经病变表型。

结局指标

340 nm吸光度变化对应的葡萄糖浓度。

血糖浓度读数。

实验动物

血液葡萄糖浓度酶法测定
组织
blood glucose assay samples
大鼠尾静脉血糖测定
组织
rat tail-vein blood
大鼠尾静脉血糖测定
组织
rat tail-vein blood
大鼠尾静脉血糖测定
组织
rat tail-vein blood
大鼠尾静脉血糖测定
组织
rat tail-vein blood
大鼠尾静脉血糖测定
组织
rat tail-vein blood

实验步骤

Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.

**Measurement of Blood Glucose ** **Measurement of Blood Glucose ** Blood samples were collected from the tail vein for the determination of blood glucose levels using Accutrend Plus test strips and meter (Roche).

模型身份

模型名称
spontaneously diabetic wbn/kob rat
物种
rat
品系
WBN/Kob

生物学特性

适用研究问题
糖尿病周围神经病变。

实验方法

实验方案

配套试剂与材料

参考文献

  1. Animal Models of Diabetic Neuropathy: Progress Since 1960s. Journal of Diabetes Research. 2013
  2. Extraction identity record 47422c59f8b82ca0b83b782c22fe751fc099b04ae7fcb71dd44dac304443e099
  3. Animal Models of Diabetic Neuropathy: Progress Since 1960s. PMID:23984428
  4. protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  5. protocols.io · vv2 · Michael Hill · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  6. ARRIVE 2.0 官方规范