跳至主要内容

high-fat diet-induced obesity mouse

高脂饮食相关肥胖及代谢负荷模型。

结局指标

340 nm吸光度变化对应的葡萄糖浓度。

实验动物

血液葡萄糖浓度酶法测定
组织
blood glucose assay samples

实验步骤

Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.

模型身份

模型名称
high-fat diet-induced obesity mouse
物种
mouse

生物学特性

适用研究问题
饮食相关肥胖与代谢表型。
模型局限
饲料组成及对照饲料的选择会影响组间比较。

实验方法

实验方案

  • 血液葡萄糖浓度酶法测定

    以己糖激酶及葡萄糖-6-磷酸脱氢酶偶联反应测定葡萄糖,使用Cobas Mira Plus读取340 nm吸光度变化。

    • 本方案对该模型的适用性

      状态:已验证适用· 证据类型:文献证据· 来源:https://doi.org/10.1021/acsomega.6c05930; https://doi.org/10.17504/protocols.io.y2kfycw

      组织:blood glucose assay samples

      血液葡萄糖浓度测定,用于糖尿病及葡萄糖稳态表征。

配套试剂与材料

参考文献

  1. Extraction identity record 4453a94ed03c5e4a6f6fe63663c5c85d432f4ff44d851f5ce45f3ad91b2aba7c
  2. Considerations and guidelines for mouse metabolic phenotyping in diabetes research. PMID:29143855
  3. protocols.io · v1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  4. ARRIVE 2.0 官方规范