high-fat diet-induced obesity Sprague-Dawley rat
Sprague-Dawley rats fed high-energy (fat and/or sugar) diets are the prevalent diet-induced obesity rat model, showing glucose intolerance, dyslipidemia and hypertension. A 45% kcal-fat diet for 8 weeks produces chronic metabolic stress with hepatic histological changes. Rats fed a 45% kcal high-fat diet rapidly gain weight, become hyperinsulinemic and develop insulin resistance in 4-6 weeks.
结局指标
血糖浓度读数。
实验动物
实验步骤
**Measurement of Blood Glucose ** **Measurement of Blood Glucose ** Blood samples were collected from the tail vein for the determination of blood glucose levels using Accutrend Plus test strips and meter (Roche).
模型身份
- 模型名称
- high-fat diet-induced obesity Sprague-Dawley rat
- 物种
- rat
- 品系
- Sprague-Dawley
生物学特性
- 适用研究问题
- Whether chronic high-fat feeding reproduces metabolic syndrome components in Sprague-Dawley rats, and how diet-induced metabolic stress affects liver regenerative capacity.
- 模型局限
- Outbred stock; the magnitude of glucose intolerance and dyslipidemia depends on diet composition and feeding duration.
参考文献
- Rat Models of Metabolic Syndrome. Methods in Molecular Biology 2019 (PMID:31228162)
- Chronic high-fat diet reprograms liver regenerative capacity in Sprague-Dawley rats (PMID:42885327)
- Alternatives to the Streptozotocin-Diabetic Rodent. Int Rev Neurobiology 2016 (PMID:27133146)
- protocols.io · vv2 · Michael Hill · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- ARRIVE 2.0 官方规范