小鼠/大鼠尿白蛋白定量（Albumin Blue 580 染料结合微量板法）
浏览实验方案用商品化 Albumin Blue 580 试剂在 96 孔微量板上以荧光读数定量小鼠或大鼠尿白蛋白浓度；含标准品系列稀释、加样、读数与标准曲线插值的完整流程。
适用范围与方法
样本：mouse or rat urine
- db/db (Lepr^db)
- 性别
- 混合
- 组织
- mouse urine
- streptozotocin-induced diabetes C57BL/6 mouse
- 性别
- 混合
- 组织
- mouse urine
- uninephrectomized db/db mouse (C57BLKS background)
- 性别
- 混合
- 组织
- mouse urine
- db/db mouse (C57BL/6 background)
- 性别
- 混合
- 组织
- mouse urine
- db/db C57BLKS mouse
- 性别
- 混合
- 组织
- mouse kidney and urine
- db/db mouse
- 性别
- 混合
- 组织
- mouse kidney and urine
- high-fat diet plus streptozotocin-induced type 2 diabetes C57BL/6J mouse
- 性别
- 混合
- 组织
- mouse urine
- ob/ob BTBR mouse
- 性别
- 混合
- 组织
- mouse urine
- streptozotocin-induced diabetes mouse
- 性别
- 混合
- 组织
- mouse urine
试剂与材料
实验流程
Prepare working reagent.
Prepare working reagent.
Prepare standards by serially diluting 200 mg/l standard 1:1 to make 100, 50, 25, 12.5, 6.25 standards.
Prepare standards by serially diluting 200 mg/l standard 1:1 to make 100, 50, 25, 12.5, 6.25 standards.
Add 25 μl of standard and sample to each well.
Add 25 μl of standard and sample to each well.
Add 125 μl of working reagent. Read in fluorimeter using 590 nm excitation and 616 nm emission.
Add 125 μl of working reagent. Read in fluorimeter using 590 nm excitation and 616 nm emission. _**IMPORTANT: **__Make sure not to add any bubbles to the wells when dispensing reagents, this will interfere with reading in the platereader._
Use a polynomial 2nd order curve fit to construct a standard curve. Interpolate the values of the unknowns using the sta
Use a polynomial 2nd order curve fit to construct a standard curve. Interpolate the values of the unknowns using the standard curve.
质量控制与输出
标准曲线用二阶多项式拟合；加样避免气泡干扰荧光读数；结果需以同批标准曲线插值。
输出为每样品尿白蛋白浓度（mg/l），按标准曲线插值报告；该读数本身不是白蛋白/肌酐比值，换算比值需另测尿肌酐。