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Il10 基因缺失自发性结肠炎模型（文献参考）

Il10 经基因靶向缺失后，多数小鼠生长迟缓、贫血并罹患慢性小肠结肠炎；肠道改变包括黏膜广泛增生、炎症反应与上皮细胞 MHC II 类分子异常表达；淋巴细胞发育与抗体应答正常。

Outcome measures

获得可供光学显微镜观察的 H&E 染色组织切片：细胞核蓝紫色、细胞质与结缔组织纤维粉红色，用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值，也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology
Age
6、10、16 周龄（来源观察时点）
Tissue
colon（结肠；来源同时观察小肠各段）
Hematoxylin and Eosin (H&E) staining for histopathology
Age
6、10、16 周龄（来源观察时点）
Tissue
colon（结肠；来源同时观察小肠各段）
Hematoxylin and Eosin (H&E) staining for histopathology
Age
6、10、16 周龄（来源观察时点）
Tissue
colon（结肠；来源同时观察小肠各段）
Hematoxylin and Eosin (H&E) staining for histopathology
Age
未记载周龄，终点取材组织切片 HE 判读（离体）
Tissue
colon（结肠；来源同时观察小肠各段）

Experimental procedures

脱蜡（Dewaxing） 石蜡切片置于二甲苯中脱蜡 3 次，每次 00:03:00。 复水（Rehydration） 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00，再置于 95% 乙醇中 1 次、00:03:00，随后用水漂洗。 苏木素核染色（Hematoxylin staining） 石蜡切片以苏木素染液染色 00:00:20，随后在流水下冲洗 00:01:00。 分化与返蓝（Differentiation and bluing） 石蜡切片置于返蓝液中 00:01:00，随后在流水下冲洗 00:01:00；此步使细胞核呈现蓝色。 伊红染色（Eosin staining） 石蜡切片以伊红复染 00:00:10；此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明（Dehydration and Clearing） 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00，随后置于二甲苯中 2 次、每次 00:02:00。 封片（Mounting） 石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name
Il10 基因缺失自发性结肠炎模型（文献参考）
Species
mouse
Taxonomy ID
10090
Genotype / zygosity
Il10 经基因靶向缺失
Allele
Il10tm1Cgn

Biological characteristics

Research questions
免疫调节缺失驱动的慢性肠道炎症机制；肠道菌群相关炎症与细胞因子失衡研究。
Limitations
文献参考模型，不代表公司实际保有、供应或质检；肠道炎症源于肠抗原刺激下不受控的免疫应答，在无特定病原（SPF）条件下突变体仅发生局限于近端结肠的局部炎症，菌群条件显著影响表型强度；不同资源品系可携带相同的 Il10tm1Cgn 等位基因，该等位基因不能单独确定动物的整体遗传背景；外推至人 IBD 需具体研究支持。

Experimental methods

Experimental protocols

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

References

  1. Kühn R, Löhler J, Rennick D, Rajewsky K, Müller W. Interleukin-10-deficient mice develop chronic enterocolitis. Cell. 1993;75(2):263-274. DOI:10.1016/0092-8674(93)80068-P; PMID:8402911. 摘要逐句支持：'generated by gene targeting'；'lymphocyte development and antibody responses are normal, but most animals are growth retarded and anemic and suffer from chronic enterocolitis'；'extensive mucosal hyperplasia, inflammatory reactions, and aberrant expression of major histocompatibility complex class II molecules on epithelia'；'mutants kept under specific pathogen-free conditions develop only a local inflammation limited to the proximal colon'；'the bowel inflammation in the mutants originates from uncontrolled immune responses stimulated by enteric antigens'；IL-10 为肠道必需的免疫调节因子。摘要未记载统一品系背景。
  2. MGI targeted allele detail: Il10<tm1Cgn>, MGI:1857199. Targeted null allele derived from E14.1 embryonic stem cells of 129P2/OlaHsd origin; original reference Kühn et al., Cell 1993, PMID:8402911. The allele is carried by multiple resource strains; its donor origin does not specify the whole genetic background of every descendant resource.
  3. NCBI Taxonomy record 10090: Mus musculus (house mouse), rank species.
  4. Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
  5. Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
  6. Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
  7. Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
  8. Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
  9. Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
  10. Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
  11. ARRIVE 2.0 guidelines
Model consultation