transient middle cerebral artery occlusion Wistar rat

记录每次定位试验的到台潜伏期和游泳轨迹，比较相继试验中的空间定位表现。到达平台与达到时限未到台的情况按对应条件路径分别处理。

存活脑组织染为红色、梗死区呈白色；以 2 mm 连续冠状切片逐孔染色成像后，测量患侧与对侧半球的梗死面积及总面积，计算患侧相对对侧的梗死面积百分比。

检查摄像机设置 检查摄像机设置。 将 Atlantis 自动升降平台置于水池，确认升降运动正常，避免平台上下移动时产生水波 将 Atlantis 自动升降平台置于水池，确认升降运动正常，避免平台上下移动时产生水波。 将平台放置在与前一天不同的位置 将平台放置在与前一天不同的位置。 实验第一天不执行与前一天位置比较的要求 实验第一天不执行与前一天位置比较的要求。 启动 Bonsai 工作流，确认以 30 fps 记录视频 启动 Bonsai 工作流，确认以 30 fps 记录视频。 降下平台，使动物暂时无法使用平台 降下平台，使动物暂时无法使用平台。 将动物放在所选起点，面向水池中心 将动物放在所选起点，面向水池中心。 轻放动物入水，避免抛掷和溅水 轻放动物入水，避免抛掷和溅水。 释放动物且操作者离开实验区域时，启动计时器或计算机跟踪程序 释放动物且操作者离开实验区域时，启动计时器或计算机跟踪程序。 经过 1 分钟 后，按下按钮升起平台 经过 1 分钟 后，按下按钮升起平台。 动物到达并接触平台时停止计时；本次试验时限为 2 分钟 Choose the matching condition: Platform — Rats will find the platform. They will do this in two minutes or less; Not platform — If the rat does not find the platform within 2 minutes. 动物到达并接触平台时停止计时；本次试验时限为 2 分钟。 返回取出动物，放入笼内擦干 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 返回取出动物，放入笼内擦干。 间隔 30 分钟 后开始第 2 次试验 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 间隔 30 分钟 后开始第 2 次试验。 升起平台，使动物能够登台，但平台仍不可直接看见 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 升起平台，使动物能够登台，但平台仍不可直接看见。 将动物放在所选起点，面向水池中心；每次试验随机选择不同的起点 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 将动物放在所选起点，面向水池中心；每次试验随机选择不同的起点。 轻放动物入水，避免抛掷和溅水 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 轻放动物入水，避免抛掷和溅水。 释放动物且操作者离开实验区域时，启动计时器或计算机跟踪程序 Condition: Platform — Rats will find the platform. They will do this in two minutes or less 释放动物且操作者离开实验区域时，启动计时器或计算机跟踪程序。 动物到达并接触平台，或经过 1 分钟 时停止计时 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Choose the matching condition: Platform — Rats will find the platform. They will do this in 1 minute or less; Not platform — If the rat does not find the platform within 1 minute 动物到达并接触平台，或经过 1 分钟 时停止计时。 返回取出动物，放入笼内擦干 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Platform — Rats will find the platform. They will do this in 1 minute or less 返回取出动物，放入笼内擦干。 间隔 5 分钟 后进行下一次试验 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Platform — Rats will find the platform. They will do this in 1 minute or less 间隔 5 分钟 后进行下一次试验。 将大鼠放在平台上 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 将大鼠放在平台上。 离开实验区域 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 离开实验区域。 等待 10 秒 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 等待 10 秒。 返回取出动物，放入笼内擦干并等待 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 返回取出动物，放入笼内擦干并等待。 间隔 5 分钟 后进行下一次试验 Condition: Platform — Rats will find the platform. They will do this in two minutes or less Condition: Not platform — If the rat does not find the platform within 1 minute 间隔 5 分钟 后进行下一次试验。 将大鼠放在平台上 Condition: Not platform — If the rat does not find the platform within 2 minutes. 将大鼠放在平台上。 离开实验区域 Condition: Not platform — If the rat does not find the platform within 2 minutes. 离开实验区域。 等待 10 秒 Condition: Not platform — If the rat does not find the platform within 2 minutes. 等待 10 秒。 返回取出动物，放入笼内擦干并等待 Condition: Not platform — If the rat does not find the platform within 2 minutes. 返回取出动物，放入笼内擦干并等待。 间隔 30 分钟 后开始第 2 次试验 Condition: Not platform — If the rat does not find the platform within 2 minutes. 间隔 30 分钟 后开始第 2 次试验。 升起平台，使动物能够登台，但平台仍不可直接看见 Condition: Not platform — If the rat does not find the platform within 2 minutes. 升起平台，使动物能够登台，但平台仍不可直接看见。 将动物放在所选起点，面向水池中心；每次试验使用不同的起点 Condition: Not platform — If the rat does not find the platform within 2 minutes. 将动物放在所选起点，面向水池中心；每次试验使用不同的起点。 轻放动物入水，避免抛掷和溅水 Condition: Not platform — If the rat does not find the platform within 2 minutes. 轻放动物入水，避免抛掷和溅水。 释放动物且操作者离开实验区域时，启动计时器或计算机跟踪程序 Condition: Not platform — If the rat does not find the platform within 2 minutes. 释放动物且操作者离开实验区域时，启动计时器或计算机跟踪程序。 动物到达并接触平台时停止计时；本次试验时限为 1 分钟 Condition: Not platform — If the rat does not find the platform within 2 minutes. 动物到达并接触平台时停止计时；本次试验时限为 1 分钟。

TTC Staining TTC Staining The brain is removed from the skull and placed into a brain slicer mold placed in ice. Razor blades are positioned in the mold to obtain 2mm thick sections. Sections are placed in individual wells of a 24 well tissue culture dish with 500 μl of 2% TTC in 0.1M PBS. The plate is wrapped in foil and placed on a 37°C slide warmer for 30 minutes. TTC is removed and 500ul of fixative is added to each well for storage. Images of the sections are captured by Olympus S2H10 research stereo- microscope and Adobe Photoshop software. The infarct area and total area of the contralateral and ipsilateral hemispheres are measured with Scion image software (Scion Corporation). The percent infarct area is calculated as a percentage of the ipsilateral hemisphere relative to the contralateral hemisphere as previously described. [F2.JPG]]()