实验方法
histopathology assay
方法说明
- 方法分类
- 组织病理
- 技术原理
- A histological assay that is intended to check for the presence or level of a specific disease.
- 输入样本
- aorta · tissue · brain tissue sections
- 可回答的研究问题
- 未提供
- 输出与判读
- 输出为图像层面的疾病程度度量（如病变面积），采样对象、放大或成像条件与判读随所采用的具体实验方案确定。
- 适用条件
- 通过组织或器官标本及其图像判断特定病变是否存在与其程度，包括主动脉 en face 斑块面积和脑组织 TTC 染色后的病变面积评价。取材、染色及面积计算采用对应具体方案。
- 局限
- 方法概念本身不提供分级阈值或统计判读标准；结果有效性依赖所采用方案规定的标定、采样与复核规程。
已发布模型
- C57 小鼠 MCAO 线栓模型
- SD 大鼠 MCAO 线栓模型
- ApoE⁻⁻ 动脉粥样硬化模型
- Ldlr⁻⁻ 动脉粥样硬化模型
- AOM/DSS 结肠炎相关结肠肿瘤模型（文献参考）
- endothelin-1 induced focal cerebral ischemia (rat)
- permanent middle cerebral artery occlusion C57BL/6 mouse
- thromboembolic stroke rat
- transient middle cerebral artery occlusion Wistar rat
- photothrombotic stroke rat
- embolic middle cerebral artery occlusion rat
- photothrombotic cortical stroke mouse
- permanent distal middle cerebral artery occlusion C57BL/6J mouse
- transient middle cerebral artery occlusion C57BL/6J mouse
- middle cerebral artery occlusion Swiss mouse
- microsphere embolic stroke rat
- intraluminal filament middle cerebral artery occlusion mouse (strain not reported)
- intraluminal filament middle cerebral artery occlusion rat (strain not reported)
- photothrombotic stroke rodent (species and strain not reported)
- thromboembolic stroke rodent (species and strain not reported)
- endothelin-1 induced focal cerebral ischemia rodent (species and strain not reported)
- permanent middle cerebral artery occlusion mouse (strain not reported)
- distal middle cerebral artery occlusion mouse (strain not reported)
- embolic middle cerebral artery occlusion mouse (strain not reported)
- middle cerebral artery occlusion rodent (surgical technique category)
- direct mechanical middle cerebral artery occlusion rat (strain not reported)
- bilateral common carotid artery occlusion combined with middle cerebral artery occlusion rat
- permanent middle cerebral artery occlusion with transient common carotid artery occlusion neonatal Wistar rat
- middle cerebral artery occlusion spontaneously hypertensive rat
- Koizumi 入路大脑中动脉阻塞模型（啮齿类）
- Zea Longa 入路大脑中动脉阻塞模型（啮齿类）
- 线栓性短暂大脑中动脉阻塞模型（啮齿类）
- 小鼠大脑中动脉局部凝血酶血栓模型
- ApoE-deficient mouse
- ApoE-deficient C57BL/6J mouse
- ApoE-deficient C57BL/6 mouse
- LDL receptor-deficient mouse
- LDL receptor-deficient C57BL/6 mouse
- LDL receptor-deficient C57BL/6J mouse
- High-fat diet-fed C57BL/6 mouse
- High-fat diet-fed mouse
- PCSK9-AAV hypercholesterolemic mouse
- ApoE3-Leiden mouse
- ApoE-deficient Fbn1 C1039G+/- mouse
已发布方案版本
- 主动脉 en face 斑块成像与面积定量
来源版本：protocols.io · vv1 · Deborah A. Howatt; Jing Liu · CC BY
- 脑切片 TTC 染色与梗死面积测定
来源版本：protocols.io · vv2 · Ian Simpson · CC BY
- 脑切片 TTC 染色与梗死面积测定
来源版本：protocols.io · vv2 · Ian Simpson · CC BY
- 小鼠主动脉根与头臂动脉取材及切片制备
来源版本：protocols.io · vv1 · Jan Breslow; Daniel Teupser · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- 结肠大体肿瘤计数、卡尺直径测量与肿瘤负荷计算
来源版本：bio-protocol · v2014-11-20 · Jalaj Gupta; Angel R. Nebreda · © 2014 The Authors; exclusive licensee Bio-protocol LLC.