high carbohydrate-high fat diet-induced rat model (cafeteria diet)
Cafeteria-style hypercaloric feeding in rats produces obesity, dyslipidemia, hyperinsulinemia, glucose intolerance, insulin resistance, hyperglycemia and inflammation.
结局指标
血糖浓度读数。
实验动物
大鼠尾静脉血糖测定
- 组织
- blood
实验步骤
**Measurement of Blood Glucose ** **Measurement of Blood Glucose ** Blood samples were collected from the tail vein for the determination of blood glucose levels using Accutrend Plus test strips and meter (Roche).
模型身份
- 模型名称
- high carbohydrate-high fat diet-induced rat model (cafeteria diet)
- 物种
- rat
生物学特性
- 表型出现阶段
- Hyperphagia and weight gain begin within days; full metabolic-syndrome picture develops over 8-16 weeks depending on diet mix.
- 适用研究问题
- Palatable-diet overconsumption, hypothalamic leptin resistance, and the most complete metabolic-syndrome spectrum among dietary rat models.
- 模型局限
- Cafeteria compositions vary widely between laboratories, making cross-study comparison difficult; intake is voluntary so caloric load is inconsistent across animals.
参考文献
- Biochemical and nutritional overview of diet-induced metabolic syndrome models in rats: what is the best choice?. Nutrition & Diabetes. 2020. PMID:32616730
- NCBI Taxonomy official record: Rattus norvegicus (brown rat), Taxonomy ID 10116.
- protocols.io · vv2 · Michael Hill · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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