TSOD mouse

TSOD mice spontaneously develop polygenic obesity with non-alcoholic fatty liver disease that progresses to steatohepatitis. Livers display mixed micro- and macrovesicular steatosis, hepatocyte ballooning, Mallory-Denk bodies, inflammatory infiltration, fibrosis, and eventual tumor development; elevated hepatic PPAR-gamma suggests excessive lipogenesis as a contributing factor.

Outcome measures

获得可供光学显微镜观察的 H&E 染色组织切片：细胞核蓝紫色、细胞质与结缔组织纤维粉红色，用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值，也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology

Age: as reported in cited studies
Tissue: liver

Experimental procedures

脱蜡（Dewaxing）石蜡切片置于二甲苯中脱蜡 3 次，每次 00:03:00。复水（Rehydration）石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00，再置于 95% 乙醇中 1 次、00:03:00，随后用水漂洗。苏木素核染色（Hematoxylin staining）石蜡切片以苏木素染液染色 00:00:20，随后在流水下冲洗 00:01:00。分化与返蓝（Differentiation and bluing）石蜡切片置于返蓝液中 00:01:00，随后在流水下冲洗 00:01:00；此步使细胞核呈现蓝色。伊红染色（Eosin staining）石蜡切片以伊红复染 00:00:10；此步使细胞质成分与结缔组织纤维呈现粉红色。脱水与透明（Dehydration and Clearing）石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00，随后置于二甲苯中 2 次、每次 00:02:00。封片（Mounting）石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name: TSOD mouse
Species: mouse
Taxonomy ID: 10090
Strain: TSOD

Biological characteristics

Phenotype stage: Steatosis and steatohepatitis features are established before 1 year of age; inflammatory infiltration, fibrosis and tumor development are reported by 1 year of age.
Research questions: Used as a polygenic model of NAFLD with obesity that recapitulates the natural course from steatosis through steatohepatitis, fibrosis and tumor development.
Limitations: The evidence adopted here for TSOD comes from a review citing the original TSOD characterization literature rather than from direct use of the model in the primary studies of this package; TSOD mice develop polygenic obesity and the specific alleles underlying the NAFLD phenotype are not defined; spontaneous tumor development after 1 year of age limits use of the model for interventional studies that require a non-tumor endpoint; genetic background substrain and precise allele composition are not reported in the sources adopted here.

Experimental methods

组织学分析

Experimental protocols

Hematoxylin and Eosin (H&E) staining for histopathology
Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

组织学二甲苯（混合异构体）
脱蜡剂
Ethanol
复水梯度溶剂（去除二甲苯）
95% 乙醇溶液
复水梯度溶剂
自来水（组织学染色用）
复水后漂洗用水（后续各流水冲洗共用）
Epredia Signature Series 苏木素染液（7221）
细胞核染色
Epredia Signature Series 返蓝液（7301）
返蓝（使细胞核转蓝）
Epredia Signature Series 伊红 Y 染液（7111）
细胞质与结缔组织纤维复染
Permount 组织学封片剂（Fisher SP15）
封片介质

References

Model consultation