TSOD mouse

TSOD mice spontaneously develop polygenic obesity with non-alcoholic fatty liver disease that progresses to steatohepatitis. Livers display mixed micro- and macrovesicular steatosis, hepatocyte ballooning, Mallory-Denk bodies, inflammatory infiltration, fibrosis, and eventual tumor development; elevated hepatic PPAR-gamma suggests excessive lipogenesis as a contributing factor.

结局指标

获得可供光学显微镜观察的 H&E 染色组织切片：细胞核蓝紫色、细胞质与结缔组织纤维粉红色，用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值，也不提供特定分子标志物的定量结果。

实验动物

Hematoxylin and Eosin (H&E) staining for histopathology

年龄: as reported in cited studies
组织: liver

实验步骤

脱蜡（Dewaxing）石蜡切片置于二甲苯中脱蜡 3 次，每次 00:03:00。复水（Rehydration）石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00，再置于 95% 乙醇中 1 次、00:03:00，随后用水漂洗。苏木素核染色（Hematoxylin staining）石蜡切片以苏木素染液染色 00:00:20，随后在流水下冲洗 00:01:00。分化与返蓝（Differentiation and bluing）石蜡切片置于返蓝液中 00:01:00，随后在流水下冲洗 00:01:00；此步使细胞核呈现蓝色。伊红染色（Eosin staining）石蜡切片以伊红复染 00:00:10；此步使细胞质成分与结缔组织纤维呈现粉红色。脱水与透明（Dehydration and Clearing）石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00，随后置于二甲苯中 2 次、每次 00:02:00。封片（Mounting）石蜡切片以 Permount 封片介质加盖玻片封固。

模型身份

模型名称: TSOD mouse
物种: mouse
NCBI Taxonomy ID: 10090
品系: TSOD

生物学特性

表型出现阶段: Steatosis and steatohepatitis features are established before 1 year of age; inflammatory infiltration, fibrosis and tumor development are reported by 1 year of age.
适用研究问题: Used as a polygenic model of NAFLD with obesity that recapitulates the natural course from steatosis through steatohepatitis, fibrosis and tumor development.
模型局限: The evidence adopted here for TSOD comes from a review citing the original TSOD characterization literature rather than from direct use of the model in the primary studies of this package; TSOD mice develop polygenic obesity and the specific alleles underlying the NAFLD phenotype are not defined; spontaneous tumor development after 1 year of age limits use of the model for interventional studies that require a non-tumor endpoint; genetic background substrain and precise allele composition are not reported in the sources adopted here.

实验方法

组织学分析

实验方案

Hematoxylin and Eosin (H&E) staining for histopathology
本方案用于固定、石蜡包埋组织切片（4 μm）的苏木素-伊红（H&E）染色：经脱蜡、复水、苏木素核染、返蓝、伊红复染、脱水透明与封片七个步骤，使细胞核呈蓝紫色、细胞质与结缔组织纤维呈粉红色，供光学显微镜下观察组织中细胞与整体结构形态。

配套试剂与材料

组织学二甲苯（混合异构体）
脱蜡剂
Ethanol
复水梯度溶剂（去除二甲苯）
95% 乙醇溶液
复水梯度溶剂
自来水（组织学染色用）
复水后漂洗用水（后续各流水冲洗共用）
Epredia Signature Series 苏木素染液（7221）
细胞核染色
Epredia Signature Series 返蓝液（7301）
返蓝（使细胞核转蓝）
Epredia Signature Series 伊红 Y 染液（7111）
细胞质与结缔组织纤维复染
Permount 组织学封片剂（Fisher SP15）
封片介质

参考文献

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