high-fat diet plus low-dose streptozotocin-induced type 2 diabetes Sprague-Dawley rat
High-fat feeding produces obesity and insulin resistance, and low-dose streptozotocin adds partial beta-cell injury, producing hyperglycaemia and glucose intolerance.
Outcome measures
340 nm吸光度变化对应的葡萄糖浓度。
血糖浓度读数。
Experimental animals
Experimental procedures
Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.
**Measurement of Blood Glucose ** **Measurement of Blood Glucose ** Blood samples were collected from the tail vein for the determination of blood glucose levels using Accutrend Plus test strips and meter (Roche).
Model identity
- Model name
- high-fat diet plus low-dose streptozotocin-induced type 2 diabetes Sprague-Dawley rat
- Species
- rat
- Strain
- Sprague-Dawley
Biological characteristics
- Limitations
- Induction depends on diet composition, streptozotocin dose and strain; it is a two-hit model, not a spontaneous form of type 2 diabetes.
Experimental methods
Experimental protocols
- 血液葡萄糖浓度酶法测定
Enzymatic blood glucose quantification by coupled hexokinase and glucose-6-phosphate dehydrogenase reactions, using absorbance change at 340 nm on the Cobas Mira Plus.
- 大鼠尾静脉血糖测定
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
- 大鼠尾静脉血糖测定
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
- 大鼠尾静脉血糖测定
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
- 大鼠尾静脉血糖测定
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
- 大鼠尾静脉血糖测定
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
Reagents and materials
- 葡萄糖酶法检测试剂（Cliniqa R84682）
葡萄糖酶法反应试剂
- 多分析物校准品（Cliniqa R60010）
分析仪校准
- 定值质控血清1（Cliniqa R83082）
质控血清1
- 定值质控血清2（Cliniqa R83083）
质控血清2
- Accutrend Plus 血糖试纸与读数仪
血糖检测耗材与读数平台
- Accutrend Plus 血糖试纸与读数仪
血糖检测耗材与读数平台
- Accutrend Plus 血糖试纸与读数仪
血糖检测耗材与读数平台
- Accutrend Plus 血糖试纸与读数仪
血糖检测耗材与读数平台
- Accutrend Plus 血糖试纸与读数仪
血糖检测耗材与读数平台
References
- Regulation of DMT1 on Bone Microstructure in Type 2 Diabetes. International Journal of Medical Sciences. 2015
- protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- protocols.io · vv2 · Michael Hill · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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