Skip to main content

Zucker fatty (fa/fa) rat

A homozygous missense mutation (Gln269Pro) in the leptin receptor gene Lepr causes defective leptin receptor signaling, producing obesity, hyperphagia and insulin resistance without leptin ligand deficiency. fa/fa rats do not develop overt diabetes and are regarded as a pre-diabetes model; they show steatosis but do not spontaneously develop NASH.

Outcome measures

血糖浓度读数。

获得可供光学显微镜观察的 H&E 染色组织切片：细胞核蓝紫色、细胞质与结缔组织纤维粉红色，用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值，也不提供特定分子标志物的定量结果。

Experimental animals

Hematoxylin and Eosin (H&E) staining for histopathology
Age
22 周龄(PMID:28953222)
Tissue
liver sections

Experimental procedures

**Measurement of Blood Glucose ** **Measurement of Blood Glucose ** Blood samples were collected from the tail vein for the determination of blood glucose levels using Accutrend Plus test strips and meter (Roche).

脱蜡（Dewaxing） 石蜡切片置于二甲苯中脱蜡 3 次，每次 00:03:00。 复水（Rehydration） 石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00，再置于 95% 乙醇中 1 次、00:03:00，随后用水漂洗。 苏木素核染色（Hematoxylin staining） 石蜡切片以苏木素染液染色 00:00:20，随后在流水下冲洗 00:01:00。 分化与返蓝（Differentiation and bluing） 石蜡切片置于返蓝液中 00:01:00，随后在流水下冲洗 00:01:00；此步使细胞核呈现蓝色。 伊红染色（Eosin staining） 石蜡切片以伊红复染 00:00:10；此步使细胞质成分与结缔组织纤维呈现粉红色。 脱水与透明（Dehydration and Clearing） 石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00，随后置于二甲苯中 2 次、每次 00:02:00。 封片（Mounting） 石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name
Zucker fatty (fa/fa) rat
Species
rat
Strain
Zucker fatty
Genotype / zygosity
Lepr fa/fa
Allele
Lepr fa (Gln269Pro missense)

Biological characteristics

Research questions
Whether leptin-receptor-deficient genetic obesity models human metabolic syndrome and prediabetes, and which stimuli are needed to progress steatosis to NASH.
Limitations
An additional stimulus is required for NASH, and the model is not considered suitable for studying macrovascular complications.

Experimental methods

Experimental protocols

  • 大鼠尾静脉血糖测定

    Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.

  • 大鼠尾静脉血糖测定

    Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.

  • Hematoxylin and Eosin (H&E) staining for histopathology

    Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

References

  1. Rat Models of Metabolic Syndrome. Methods in Molecular Biology 2019 (PMID:31228162)
  2. Leptin- and Leptin Receptor-Deficient Rodent Models: Relevance for Human Type 2 Diabetes (PMID:24809394)
  3. Animal Models of Nonalcoholic Fatty Liver Disease - A Starter's Guide (PMID:28953222)
  4. Alternatives to the Streptozotocin-Diabetic Rodent. Int Rev Neurobiology 2016 (PMID:27133146)
  5. protocols.io · vv2 · Michael Hill · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  6. Nathalia L. Oliveira; Samantha Gruenheid. Hematoxylin and Eosin (H&E) staining for histopathology. protocols.io, v1, 2026-07-31. DOI: 10.17504/protocols.io.kxygxr9jdg8j
  7. Epredia Signature Series Hematoxylin (7221), 产品信息页, Fisher Scientific
  8. Epredia Signature Series Bluing Reagent (7301), 产品信息页, Fisher Scientific
  9. Epredia Signature Series Eosin Y (7111), 产品信息页, Fisher Scientific
  10. Xylene, Fisherbrand HistoPrep (HC7001GAL), 产品信息页, Fisher Scientific
  11. Fisher Chemical Permount Mounting Medium (SP15 产品族), 产品信息页, Fisher Scientific
  12. Ethanol (CID 702, CAS 64-17-5), PubChem 化合物标准记录
  13. ARRIVE 2.0 guidelines
Model consultation