High-fat diet-induced NASH Sprague-Dawley rat

Male Sprague-Dawley rats fed a high-fat diet containing approximately 60 to 71 kcal percent fat develop hepatocellular steatosis with hepatocyte ballooning injury and elevated hepatic triglycerides over 6 to 12 weeks. When combined with a single low-dose streptozotocin injection or initiated by diethylnitrosamine, the same diet drives more severe NASH-like inflammation and accelerates hepatocarcinogenesis, with NAS typically in the 5 to 7 range under methionine-choline-deficient regimens and lower NAS under high fat alone.

Outcome measures

血糖浓度读数。

获得可供光学显微镜观察的 H&E 染色组织切片：细胞核蓝紫色、细胞质与结缔组织纤维粉红色，用于观察组织中细胞的整体结构与形态细节。本方案本身不产出病理评分数值，也不提供特定分子标志物的定量结果。

Experimental animals

大鼠尾静脉血糖测定

Age: as reported in cited studies
Tissue: blood

Hematoxylin and Eosin (H&E) staining for histopathology

Age: as reported in cited studies
Tissue: liver

Experimental procedures

**Measurement of Blood Glucose ** **Measurement of Blood Glucose ** Blood samples were collected from the tail vein for the determination of blood glucose levels using Accutrend Plus test strips and meter (Roche).

脱蜡（Dewaxing）石蜡切片置于二甲苯中脱蜡 3 次，每次 00:03:00。复水（Rehydration）石蜡切片依次置于 100% 乙醇中 3 次、每次 00:03:00，再置于 95% 乙醇中 1 次、00:03:00，随后用水漂洗。苏木素核染色（Hematoxylin staining）石蜡切片以苏木素染液染色 00:00:20，随后在流水下冲洗 00:01:00。分化与返蓝（Differentiation and bluing）石蜡切片置于返蓝液中 00:01:00，随后在流水下冲洗 00:01:00；此步使细胞核呈现蓝色。伊红染色（Eosin staining）石蜡切片以伊红复染 00:00:10；此步使细胞质成分与结缔组织纤维呈现粉红色。脱水与透明（Dehydration and Clearing）石蜡切片依次置于 95% 乙醇中 00:02:00、100% 乙醇中 2 次、每次 00:02:00，随后置于二甲苯中 2 次、每次 00:02:00。封片（Mounting）石蜡切片以 Permount 封片介质加盖玻片封固。

Model identity

Model name: High-fat diet-induced NASH Sprague-Dawley rat
Species: rat
Taxonomy ID: 10116
Strain: Sprague-Dawley
Genetic background: Sprague-Dawley

Biological characteristics

Phenotype stage: Male Sprague-Dawley rats, typically 8 weeks old and 180 to 200 g at start, are fed the high-fat diet for 6 to 12 weeks; diethylnitrosamine-initiated carcinogenesis protocols add a single priming injection followed by 6 weeks of high-fat feeding.
Research questions: The model has been used to evaluate hepatoprotective natural products such as lycopene, tomato extract and Dendrobium officinale polysaccharide against high-fat diet-induced MAFLD, and to compare NAFL, NASH and NAFL-plus-type-2-diabetes phenotypes across high-fat, methionine-choline-deficient and high-fat-plus-streptozotocin regimens in Sprague-Dawley rats.
Limitations: High-fat diet alone in Sprague-Dawley rats produces steatosis with modest NAS but limited progressive fibrosis over 12 weeks, so claims of advanced NASH require combination with methionine-choline deficiency or a carcinogen primer. The corpus does not document long-term fibrosis or cirrhosis endpoints, and diet compositions vary between studies, which limits cross-study comparison.

Experimental methods

Experimental protocols

大鼠尾静脉血糖测定
Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.
Hematoxylin and Eosin (H&E) staining for histopathology
Hematoxylin and eosin (H&E) staining of fixed, paraffin-embedded tissue sections (4 μm). The workflow covers dewaxing, rehydration, hematoxylin nuclear staining, bluing, eosin counterstaining, dehydration and clearing, and coverslipping, yielding blue-purple nuclei and pink cytoplasm and connective tissue fibers for light-microscopic assessment of cellular and tissue morphology.

Reagents and materials

Accutrend Plus 血糖试纸与读数仪
血糖检测耗材与读数平台
组织学二甲苯（混合异构体）
脱蜡剂
Ethanol
复水梯度溶剂（去除二甲苯）
95% 乙醇溶液
复水梯度溶剂
自来水（组织学染色用）
复水后漂洗用水（后续各流水冲洗共用）
Epredia Signature Series 苏木素染液（7221）
细胞核染色
Epredia Signature Series 返蓝液（7301）
返蓝（使细胞核转蓝）
Epredia Signature Series 伊红 Y 染液（7111）
细胞质与结缔组织纤维复染
Permount 组织学封片剂（Fisher SP15）
封片介质

References

Model consultation