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血液葡萄糖浓度酶法测定

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Enzymatic blood glucose quantification by coupled hexokinase and glucose-6-phosphate dehydrogenase reactions, using absorbance change at 340 nm on the Cobas Mira Plus.

Scope and method

Sample：血液葡萄糖检测样本，按Cobas Mira Plus相应分析流程处理

葡萄糖测定

Reagents and materials

Procedure

  1. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum.

    Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum.

  2. Sample Handling as performed by the Cobas Mira Plus.

    Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.

Quality control and readouts

以多分析物校准品和两种定值质控血清进行校准与质控。

340 nm吸光度变化对应的葡萄糖浓度。

References

  1. protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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