ob/ob C57BL/6 mouse
Lep ob/ob突变相关严重肥胖表型。
Outcome measures
340 nm吸光度变化对应的葡萄糖浓度。
Experimental animals
- Tissue
- blood glucose assay samples
Experimental procedures
Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.
Model identity
- Model name
- ob/ob C57BL/6 mouse
- Species
- mouse
- Strain
- C57BL/6
- Genotype / zygosity
- Lep ob/ob
- Allele
- Lep ob
Biological characteristics
- Research questions
- 瘦素缺陷相关肥胖与代谢异常。
Experimental methods
Experimental protocols
- 血液葡萄糖浓度酶法测定
Enzymatic blood glucose quantification by coupled hexokinase and glucose-6-phosphate dehydrogenase reactions, using absorbance change at 340 nm on the Cobas Mira Plus.
Reagents and materials
- 葡萄糖酶法检测试剂（Cliniqa R84682）
葡萄糖酶法反应试剂
- 多分析物校准品（Cliniqa R60010）
分析仪校准
- 定值质控血清1（Cliniqa R83082）
质控血清1
- 定值质控血清2（Cliniqa R83083）
质控血清2
References
- Future Perspective of Diabetic Animal Models. Endocrine, Metabolic & Immune Disorders Drug Targets. 2020
- Extraction identity record 176c76e911e37a881650023bcfadac066a96f2565e402222fb901c3890389790
- Future Perspective of Diabetic Animal Models. PMID:31241444
- protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- ARRIVE 2.0 guidelines