high-fat diet-induced obesity mouse
高脂饮食相关肥胖及代谢负荷模型。
Outcome measures
340 nm吸光度变化对应的葡萄糖浓度。
Experimental animals
- Tissue
- blood glucose assay samples
Experimental procedures
Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum. Sample Handling as performed by the Cobas Mira Plus. Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for 10 minutes. e) Absorbance is measured at 340 nm. Change in absorbance is calculated.
Model identity
- Model name
- high-fat diet-induced obesity mouse
- Species
- mouse
Biological characteristics
- Research questions
- 饮食相关肥胖与代谢表型。
- Limitations
- 饲料组成及对照饲料的选择会影响组间比较。
Experimental methods
Experimental protocols
- 血液葡萄糖浓度酶法测定
Enzymatic blood glucose quantification by coupled hexokinase and glucose-6-phosphate dehydrogenase reactions, using absorbance change at 340 nm on the Cobas Mira Plus.
Reagents and materials
- 葡萄糖酶法检测试剂（Cliniqa R84682）
葡萄糖酶法反应试剂
- 多分析物校准品（Cliniqa R60010）
分析仪校准
- 定值质控血清1（Cliniqa R83082）
质控血清1
- 定值质控血清2（Cliniqa R83083）
质控血清2
References
- Extraction identity record 4453a94ed03c5e4a6f6fe63663c5c85d432f4ff44d851f5ce45f3ad91b2aba7c
- Considerations and guidelines for mouse metabolic phenotyping in diabetes research. PMID:29143855
- protocols.io · vv1 · John Stack; Gary Cline · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- ARRIVE 2.0 guidelines