Sterile microcentrifuge tube for cDNA synthesis

Quality standard

The source explicitly specifies a sterile microcentrifuge tube but does not report tube capacity or the total required per experiment.

Use information

Prepare the brain-tissue RNA, primer and dNTP mixture for first-strand cDNA synthesis.

References

1. protocols.io v2; step protocolsio-893163; verbatim source: In a sterile microfuge tube add:

Used in these protocol BOMs

• First-strand cDNA synthesis from brain tissue RNAprotocol #187 · revision 3

  Source version：protocols.io · v2 · New England Biolabs · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

  Reverse transcription and cDNA synthesis

  This reagent's uses & amounts

  • Prepare the RNA and primer mixture · Prepare the brain-tissue RNA, primer and dNTP mixture for first-strand cDNA synthesis. · Not reported in the source · The source explicitly specifies a sterile microcentrifuge tube but does not report tube capacity or the total required per experiment.

  Associated models: 11 protocol→model record(s)1 verified · 0 candidate

  Model → research topic：1 verified · 0 candidate。These statuses apply to individual relationships, not to this reagent.

  Recorded use and applicability limits

  • Protocol → model：Wistar ICV-STZ rat：

    Reverse-transcribe total RNA from brain tissue into cDNA as input for Sirt1/GAPDH real-time quantitative PCR.

  • Protocol → model：Wistar ICV-STZ rat：

    Age：3–4 months of age

  • Protocol → model：Wistar ICV-STZ rat：

    Sex：Male

  • Protocol → model：Wistar ICV-STZ rat：

    Tissue：Brain tissue-derived total RNA

  • Model → research topic：Wistar ICV-STZ rat → Alzheimer disease：

    Study the connections among brain energy metabolism, neural injury, and cognitive function, and evaluate the effects of candidate interventions on the relevant phenotypes.

  Show 1 model association(s)

  Wistar ICV-STZ rat · Verified applicability

  This protocol's applicability to this model

  Status：Verified applicability· Evidence type：Literature evidence· Source：PMID: 32336744; DOI: 10.1538/expanim.19-0146. The Real-time quantitative PCR section records the detection of rat brain tissue RNA→cDNA→Sirt1/GAPDH. The protocols.io source used provides the complete workflow for this stage; the TRIpure/Super MMLV used in that study differs from the TRIzol/PureLink/ProtoScript II reagent system used in this instance, and no claim is made of in-house validation or consistency with the original study parameters.

  Age：3–4 months of ageSex：MaleTissue：Brain tissue-derived total RNA

  Reverse-transcribe total RNA from brain tissue into cDNA as input for Sirt1/GAPDH real-time quantitative PCR.

  Related research topics: 1 (1 verified · 0 candidate)

  • · Alzheimer disease · Verified applicability

    This model's applicability to this disease

    Status：Verified applicability· Evidence type：Literature evidence· Source：https://doi.org/10.1538/expanim.19-0146

    Study the connections among brain energy metabolism, neural injury, and cognitive function, and evaluate the effects of candidate interventions on the relevant phenotypes.

Reference, supply and purchase path

No verified catalog mapping exists for this reagent; reference status: unknown.

No listed sale configuration is published here. This states neither discontinuation nor current stock.