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Technical services

Histopathology

Examine tissue pathology with section staining and immunohistochemistry.

Histopathological section staining

Observe tissue morphology and lesions, including plaque section staining in atherosclerosis research.

IHC

Immunohistochemistry to examine the distribution of target markers in tissue.

Molecular assays

Explore gene expression and protein measures with qPCR, ELISA and WB.

qPCR

Quantitative PCR analysis to measure target gene expression.

ELISA

Enzyme-linked immunosorbent analysis to measure target proteins in samples.

Western blot (WB)

Western blot analysis to examine target protein expression.

Multiomics

Explore molecular changes in tissues and cells through transcriptomic data in model research.

Single-cell transcriptomics

Analyze gene expression at single-cell resolution, including hippocampal tissue in Alzheimer’s disease model research.

Sample requirements, assay scope, deliverables and current availability are confirmed for each research project.

Experimental methods

Experimental protocols

Protocols apply a method to a defined measurement, with samples, procedures and quality controls. Available services are linked separately.

  • 小鼠主动脉根与头臂动脉病变油红 O 染色及面积定量

    Version 3

    Collect the mouse heart-aorta preparation and brachiocephalic artery, fix, embed in gelatin and prepare frozen sections; stain lipids with Oil Red O, image the sections and quantify lesion area at the aortic root and brachiocephalic artery.

  • 小鼠主动脉根与头臂动脉取材及切片制备

    Version 3

    Flush the mouse circulation with PBS, collect the heart-aorta preparation and brachiocephalic artery, and fix or OCT-embed them for section-based quantification of atherosclerosis at the aortic sinus and brachiocephalic artery.

  • 大鼠尾静脉血糖测定

    Version 2

    Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.

  • 大鼠尾静脉血糖测定

    Version 1

    Measure rat tail-vein blood glucose using Roche Accutrend Plus test strips and meter.

  • 血液葡萄糖浓度酶法测定

    Version 1

    Enzymatic blood glucose quantification by coupled hexokinase and glucose-6-phosphate dehydrogenase reactions, using absorbance change at 340 nm on the Cobas Mira Plus.

  • 小鼠胰岛素耐量试验（ITT）

    Version 2

    This protocol adopts the protocols.io Insulin Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given insulin at 1–1.2 U/kg by intraperitoneal injection. Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after the load to assess insulin sensitivity. The record describes the assay procedure, not executed disease-model results.

  • 小鼠口服葡萄糖耐量试验（OGTT）

    Version 1

    This protocol adopts the protocols.io Oral Glucose Tolerance Test in Mouse v1. Mice are fasted for 4–6 hours, weighed, and given oral glucose at 1 g/kg (0.5 g/kg after at least 4 weeks of high-fat diet). Blood glucose is measured before and at 15, 30, 60, 90 and 120 minutes after the load, with tail-vein blood collected at selected time points for plasma. The record describes the assay procedure, not executed disease-model results.

  • 小鼠 Morris 水迷宫空间学习与记忆测定

    Version 10

    Assess spatial learning and memory in adult mice using hidden-platform training and platform-free probe trials, with escape latency, path length, swim velocity and target-quadrant time as readouts.

  • 脑切片 TTC 染色与梗死面积测定

    Version 9

    Fresh brains are cut to 2-mm coronal sections, stained per well with 2% TTC in 0.1M PBS at 37°C for 30 min protected from light, stored in fixative, imaged on a stereomicroscope, and infarct percentage computed ipsilateral-versus-contralateral.

  • 小鼠 Morris 水迷宫空间学习与记忆测定

    Version 9

    Assess spatial learning and memory in adult mice using hidden-platform training and platform-free probe trials, with escape latency, path length, swim velocity and target-quadrant time as readouts.