跳至主要内容

ldl receptor deficiency with loss of apob mrna editing mouse

Ldlr 与 Apobec1 双缺失小鼠缺乏 ApoB mRNA 编辑并产生 ApoB100,用于研究富含 ApoB100 的脂蛋白与高胆固醇血症。

结局指标

获得可用于兔主动脉斑块组织学分级和组成的 HE 染色切片。

实验动物

动脉组织石蜡切片 H&E 染色
年龄
不限(固定组织切片)
组织
动脉组织

实验步骤

H&E staining **Dewaxing:** Paraffin sections are placed in xylene for 3 x 00:03:00 H&E staining **Rehydration: ** Paraffin sections are placed in - 100% EtOH for 3 x 00:03:00 - 95% EtOH for 1 x 00:03:00 - Rinse with water H&E staining **Hematoxylin staining: ** Paraffin sections are stained with hematoxylin for 00:00:20 and rinse under running water for 00:01:00 H&E staining **Differentiation and bluing:** Paraffin sections are placed into the bluing reagent for 00:01:00 and rinse under running water for 00:01:00 _This step results in a blue coloration of the nuclei._ H&E staining **Eosin staining:** Parrafin sections are counterstained with Eosin for 00:00:10 _This step results in a pink coloration of the cytoplasmic components and connective tissue fibers._ H&E staining **Dehydration and Clearing:** Paraffin sections are placed in - 95% EtOH for 00:02:00 - 100% EtOH for 2 x 00:02:00 - Xylene for 2 x 00:02:00 H&E staining **Mounting:** Parrafin sections are mounted with a glass coverslip using Permount.

模型身份

模型名称
ldl receptor deficiency with loss of apob mrna editing mouse
物种
mouse
基因型/合子状态
Ldlr −/−; Apobec1 −/−

实验方法

实验方案

配套试剂与材料

参考文献

  1. Mouse Models of Atherosclerosis: What They Teach Us, What They Miss, and When to Use Them Bioengineering 2026 PMID:42649799
  2. Mouse Models of Atherosclerosis: What They Teach Us, What They Miss, and When to Use Them PMID:42649799
  3. protocols.io · vv1 · Nathalia L. Oliveira; samantha.gruenheid · This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
  4. ARRIVE 2.0 官方规范