beta cell-specific Tcf7l2 knockout mouse (RIP2.Cre-ER T2, tamoxifen-inducible)

Beta-cell Tcf7l2 knockout mice on normal or high fat diet displayed normal glucose tolerance when glucose was introduced by the intraperitoneal route.

Outcome measures

以血糖浓度对时间作图得到完整血糖-时间曲线，并可计算曲线下面积（AUC）反映葡萄糖清除程度；当各受试对象基线血糖不同时应比较高于基线的幅度或曲线下面积，而非仅比较单一终点值。该检测反映葡萄糖负荷后的整体清除，不等同于胰岛素作用的直接测量。

Experimental animals

小鼠腹腔注射葡萄糖耐量检测（IPGTT）

Tissue: blood

Experimental procedures

提前一周单笼饲养实验前一周将小鼠改为单笼饲养。称量体重逐只称量小鼠体重，用于按体重计算给糖体积。禁食6小时禁食六小时（06:00:00）。测定基线血糖禁食结束后，先用血糖仪测定血糖作为基线。腹腔注射葡萄糖按2 g/kg体重腹腔注射葡萄糖：将2 g D-葡萄糖溶于10 ml生理盐水配成20%葡萄糖贮备液，按每克体重10 ul给予。系列采血测定血糖于注射葡萄糖后15、30、45、60、90和120分钟采集血样，用血糖仪测定血糖。

Model identity

Model name: beta cell-specific Tcf7l2 knockout mouse (RIP2.Cre-ER T2, tamoxifen-inducible)
Species: mouse
Genotype / zygosity: Tcf7l2 floxed exon 10 with tamoxifen-inducible RIP2.Cre-ER T2

Biological characteristics

Research questions: Whether beta cell-specific Tcf7l2 deletion reproduces the islet phenotype implied by human GWAS.
Limitations: Inducible deletion plus intraperitoneal challenge can mask secretory defects; a normal IPGTT does not exclude beta-cell dysfunction seen by other tests.

Experimental methods

葡萄糖耐量试验

Experimental protocols

小鼠腹腔注射葡萄糖耐量检测（IPGTT）
Intraperitoneal glucose tolerance test (IPGTT) in mice: single-house for one week, weigh, fast for 6 hours, measure baseline glucose, inject 2 g/kg body weight of 20% D-glucose intraperitoneally, then collect blood at 15 to 120 minutes after injection. Used to assess glucose clearance in obesity and glucose-metabolism models.

Reagents and materials

0.9% 氯化钠溶液（生理盐水）
葡萄糖负荷液的溶剂/载体
D-葡萄糖（D-glucose）
葡萄糖负荷溶质

References

Model consultation