beta cell-specific ZnT8 knockout mouse (RIP2 promoter)

Deletion of ZnT8 selectively from beta cells reproduces effects on glucose homeostasis similar to the systemic knockout.

Outcome measures

以血糖浓度对时间作图得到完整血糖-时间曲线，并可计算曲线下面积（AUC）反映葡萄糖清除程度；当各受试对象基线血糖不同时应比较高于基线的幅度或曲线下面积，而非仅比较单一终点值。该检测反映葡萄糖负荷后的整体清除，不等同于胰岛素作用的直接测量。

Experimental animals

小鼠腹腔注射葡萄糖耐量检测（IPGTT）

Tissue: blood

Experimental procedures

提前一周单笼饲养实验前一周将小鼠改为单笼饲养。称量体重逐只称量小鼠体重，用于按体重计算给糖体积。禁食6小时禁食六小时（06:00:00）。测定基线血糖禁食结束后，先用血糖仪测定血糖作为基线。腹腔注射葡萄糖按2 g/kg体重腹腔注射葡萄糖：将2 g D-葡萄糖溶于10 ml生理盐水配成20%葡萄糖贮备液，按每克体重10 ul给予。系列采血测定血糖于注射葡萄糖后15、30、45、60、90和120分钟采集血样，用血糖仪测定血糖。

Model identity

Model name: beta cell-specific ZnT8 knockout mouse (RIP2 promoter)
Species: mouse
Genotype / zygosity: ZnT8 deletion driven by the RIP2 promoter

Biological characteristics

Research questions: Whether beta cell ZnT8 deletion accounts for the systemic knockout phenotype.
Limitations: Phenotype attribution is specific to the beta-cell line; the RIP2 transgene expression pattern should be verified against the systemic model used for comparison.

Experimental methods

葡萄糖耐量试验

Experimental protocols

小鼠腹腔注射葡萄糖耐量检测（IPGTT）
Intraperitoneal glucose tolerance test (IPGTT) in mice: single-house for one week, weigh, fast for 6 hours, measure baseline glucose, inject 2 g/kg body weight of 20% D-glucose intraperitoneally, then collect blood at 15 to 120 minutes after injection. Used to assess glucose clearance in obesity and glucose-metabolism models.

Reagents and materials

0.9% 氯化钠溶液（生理盐水）
葡萄糖负荷液的溶剂/载体
D-葡萄糖（D-glucose）
葡萄糖负荷溶质

References

Model consultation